To advance the translation of quantitative proteomics into the clinic, we are comparing the performance of the leading commercial nanoflow liquid chromatography (nLC) system (based on literature reviews), the Easy-nLC 1200 (Thermo Fisher Scientific, Waltham, MA, U.S.A.), to the Evosep One HPLC (Evosep Biosystems, Odense, Denmark). We measured FFPE-tissue digests from 21 biological replicates with a similar gradient on both of the LC systems while keeping the on-column amount (1 µg total protein) and the single-shot data-dependent acquisition-based MS/MS method constant.
Overall, the Evosep One facilitates robust and sensitive high-throughput sample acquisition, making it suitable for clinical MS. We found the Evosep One to be a useful platform for positioning mass spectrometry-based proteomics in the clinical setting. The clinical application of nLC/MS will inform clinical decision-making in oncology and other diseases.
■为了推进定量蛋白质组学在临床中的应用,我们正在比较领先的商用纳米低液相色谱(nLC)系统的性能(基于文献综述),Easy-nLC1200(ThermoFisherScientific,沃尔瑟姆,MA,U.S.A.),到EvosepOneHPLC(EvosepBiosystems,欧登塞,丹麦)。我们在两个LC系统上以相似的梯度测量了21个生物重复的FFPE组织消化物,同时保持柱上量(1µg总蛋白)和基于单次数据依赖性采集的MS/MS方法恒定。
■总的来说,EvosepOne有助于稳健和灵敏的高通量样品采集,使其适合临床MS。我们发现EvosepOne是在临床环境中定位基于质谱的蛋白质组学的有用平台。nLC/MS的临床应用将为肿瘤学和其他疾病的临床决策提供信息。