Abstract:
Fermentable oligo-, di-, monosaccharides and polyols (FODMAPs) have emerged as key contributors to digestive discomfort and intolerance to certain vegetables, fruits, and plant-based foods. Although strategies exist to minimize FODMAP consumption and exposure, exogenous enzyme supplementation targeting the fructan-type FODMAPs has been underexploited. The objective of this study was to test the hydrolytic efficacy of a food-grade, non-genetically engineered microbial inulinase preparation toward inulin-type fructans in the INFOGEST in vitro static simulation of gastrointestinal (GI) digestion. Purified inulin was shown to undergo acid-mediated hydrolysis at high gastric acidity as well as predominantly inulinase-mediated hydrolysis at lower gastric acidity. Inulinase dose-response simulations of inulin, garlic, and high-fructan meal digestion in the gastric phase suggest that as little as 50 inulinase units (INU) and up to 800 INU per serving promote fructan hydrolysis better than the control simulations without inulinase. Liquid chromatography-mass spectrometry (LC-MS) profiling of fructo-oligosaccharides (FOS) in the gastric digestas following inulinase treatment confirms the fructolytic activity of inulinase under simulated digestive conditions. Altogether, these in vitro digestion data support the use of microbial inulinase as an exogenous enzyme supplement for reducing dietary fructan-type FODMAP exposure.
摘要:
可发酵寡头,di-,单糖和多元醇(FODMAPs)已成为消化不适和对某些蔬菜不耐受的关键贡献者,水果,和植物性食品。尽管存在最小化FODMAP消耗和暴露的策略,针对果聚糖型FODMAP的外源酶补充剂尚未得到充分利用。这项研究的目的是测试食品级的水解功效,非基因工程微生物菊粉酶制剂中的菊粉型果聚糖的INFOGEST体外静态模拟胃肠道(GI)消化。纯化的菊粉在高胃酸度下会经历酸介导的水解,而在较低胃酸度下主要是菊粉酶介导的水解。菊粉的菊粉酶剂量反应模拟,大蒜,大蒜和胃期的高果聚糖粉消化表明,每份仅50个菊粉酶单位(INU)和多达800个INU比没有菊粉酶的对照模拟更好地促进果聚糖水解。菊粉酶处理后胃消化物中低聚果糖(FOS)的液相色谱-质谱(LC-MS)分析证实了菊粉酶在模拟消化条件下的果糖分解活性。总之,这些体外消化数据支持使用微生物菊粉酶作为外源酶补充剂来减少膳食中果聚糖型FODMAP暴露.