Abstract：
Improvement of cell migration by the nano-topographical modification of implant surface can directly or indirectly accelerate wound healing and osseointegration between bone and implant. Therefore, modification of the implant surface was done with TiO2 nanorod (NR) arrays to develop a more osseointegration-friendly implant in this study. Modulating the migration of a cell, adhered to a scaffold, by the variations of NR diameter, density and tip diameter in vitro is the primary objective of the study. The fluid structure interaction method was used, followed by the submodelling technique in this multiscale analysis. After completing a simulation over a global model, fluid structure interaction data was applied to the sub-scaffold finite element model to predict the mechanical response over cells at the cell-substrate interface. Special focus was given to strain energy density at the cell interface as a response parameter due to its direct correlation with the migration of an adherent cell. The results showed a huge rise in strain energy density after the addition of NRs on the scaffold surface. It also highlighted that variation in NR density plays a more effective role than the variation in NR diameter to control cell migration over a substrate. However, the effect of NR diameter becomes insignificant when the NR tip was considered. The findings of this study could be used to determine the best nanostructure parameters for better osseointegration.
摘要：
通过植入物表面的纳米形貌修饰来改善细胞迁移可以直接或间接地加速骨与植入物之间的伤口愈合和骨整合。因此,在这项研究中，使用TiO2纳米棒（NR）阵列对植入物表面进行了修饰，以开发对骨整合更友好的植入物。调节细胞的迁移，粘附在脚手架上，通过NR直径的变化，体外密度和尖端直径是研究的主要目标。采用流体结构相互作用法，其次是这种多尺度分析中的子建模技术。在全局模型上完成仿真后，将流体结构相互作用数据应用于子支架有限元模型，以预测细胞-基底界面处细胞的力学响应。由于其与贴壁细胞的迁移直接相关，因此特别关注细胞界面处的应变能密度作为响应参数。结果表明，在支架表面添加NRs后，应变能密度大幅上升。它还强调了NR密度的变化在控制细胞在基质上的迁移方面比NR直径的变化起更有效的作用。然而,当考虑NR尖端时，NR直径的影响变得微不足道。这项研究的结果可用于确定最佳的纳米结构参数，以实现更好的骨整合。
