PDF(Pubmed)
Abstract：
A conditioning lesion of the peripheral sensory axon triggers robust central axon regeneration in mammals. We trigger conditioned regeneration in the Caenorhabditis elegans ASJ neuron by laser surgery or genetic disruption of sensory pathways. Conditioning upregulates thioredoxin-1 (trx-1) expression, as indicated by trx-1 promoter-driven expression of green fluorescent protein and fluorescence in situ hybridization (FISH), suggesting trx-1 levels and associated fluorescence indicate regenerative capacity. The redox activity of trx-1 functionally enhances conditioned regeneration, but both redox-dependent and -independent activity inhibit non-conditioned regeneration. Six strains isolated in a forward genetic screen for reduced fluorescence, which suggests diminished regenerative potential, also show reduced axon outgrowth. We demonstrate an association between trx-1 expression and the conditioned state that we leverage to rapidly assess regenerative capacity.
摘要：
外周感觉轴突的调节损伤会触发哺乳动物强大的中央轴突再生。我们通过激光手术或感觉通路的遗传破坏在C.elegansASJ神经元中触发条件再生。调理上调硫氧还蛋白-1(trx-1)表达，如trx-1启动子驱动的绿色荧光蛋白表达和荧光原位杂交所示，表明trx-1水平和相关的荧光表明再生能力。trx-1的氧化还原活性在功能上增强了条件再生，但氧化还原依赖性和非依赖性活性均抑制非条件性再生。在正向遗传筛选中分离出6个菌株，用于减少荧光，这表明再生潜力下降，也显示轴突生长减少。我们证明了trx-1表达与我们用来快速评估再生能力的条件状态之间的关联。
