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Abstract：
Fungi are an important group of microorganisms that play crucial roles in a variety of ecological and biotechnological processes. Fungi depend on intracellular protein trafficking, which involves moving proteins from their site of synthesis to the final destination within or outside the cell. The soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins are vital components of vesicle trafficking and membrane fusion, ultimately leading to the release of cargos to the target destination. The v-SNARE (vesicle-associated SNARE) Snc1 is responsible for anterograde and retrograde vesicle trafficking between the plasma membrane (PM) and Golgi. It allows for the fusion of exocytic vesicles to the PM and the subsequent recycling of Golgi-localized proteins back to the Golgi via three distinct and parallel recycling pathways. This recycling process requires several components, including a phospholipid flippase (Drs2-Cdc50), an F-box protein (Rcy1), a sorting nexin (Snx4-Atg20), a retromer submit, and the COPI coat complex. Snc1 interacts with exocytic SNAREs (Sso1/2, Sec9) and the exocytic complex to complete the process of exocytosis. It also interacts with endocytic SNAREs (Tlg1 and Tlg2) during endocytic trafficking. Snc1 has been extensively investigated in fungi and has been found to play crucial roles in various aspects of intracellular protein trafficking. When Snc1 is overexpressed alone or in combination with some key secretory components, it results in enhanced protein production. This article will cover the role of Snc1 in the anterograde and retrograde trafficking of fungi and its interactions with other proteins for efficient cellular transportation.
摘要：
真菌是一类重要的微生物，在各种生态和生物技术过程中起着至关重要的作用。真菌依赖于细胞内蛋白质运输，这涉及将蛋白质从其合成位点转移到细胞内外的最终目的地。可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）蛋白是囊泡运输和膜融合的重要组成部分，最终导致货物释放到目标目的地。v-SNARE（囊泡相关的SNARE）Snc1负责质膜（PM）和高尔基体之间的顺行和逆行囊泡运输。它允许胞外囊泡与PM融合，并随后通过三种不同且平行的再循环途径将高尔基体定位的蛋白质再循环回高尔基体。这个回收过程需要几个组件，包括磷脂翻转酶（Drs2-Cdc50），一种F盒蛋白（Rcy1），排序Nexin(Snx4-Atg20)，回溯提交，和COPI外套综合体。Snc1与胞吐SNARE（Sso1/2，Sec9）和胞吐复合物相互作用，完成胞吐过程。它还在内吞运输期间与内吞SNARE(Tlg1和Tlg2)相互作用。Snc1已在真菌中进行了广泛的研究，并已发现在细胞内蛋白质运输的各个方面发挥关键作用。当Snc1单独或与一些关键的分泌成分组合过表达时，它导致蛋白质产量增加。本文将介绍Snc1在真菌的顺行和逆行运输中的作用及其与其他蛋白质的相互作用，以实现有效的细胞运输。
