PDF(Pubmed)
Abstract：
Poor diet quality is a risk factor for type 2 diabetes and cardiovascular disease. However, knowledge of metabolites marking adherence to Dietary Guidelines for Americans (2015 version) are limited.
The goal was to determine a pattern of metabolites associated with the Healthy Eating Index (HEI)-2015, which measures adherence to the Dietary Guidelines for Americans.
The analysis examined 3557 adult men and women from the longitudinal cohort Multiethnic Study of Atherosclerosis (MESA), without known cardiovascular disease and with complete dietary data. Fasting serum specimens and diet and demographic questionnaires were assessed at baseline. Untargeted 1H 1-dimensional nuclei magnetic resonance spectroscopy (600 MHz) was used to generate metabolomics and lipidomics. A metabolome-wide association study specified each spectral feature as outcomes, HEI-2015 score as predictor, adjusting for age, sex, race, and study site in linear regression analyses. Subsequently, hierarchical clustering defined the discrete groups of correlated nuclei magnetic resonance features associated with named metabolites, and the linear regression analysis assessed for associations with HEI-2015 total and component scores.
The sample included 50% women with an mean age of 63 years, with 40% identifying as White, 23% as Black, 24% as Hispanic, and 13% as Chinese American. The mean HEI-2015 score was 66. The metabolome-wide association study identified 179 spectral features significantly associated with HEI-2015 score. The cluster analysis identified 7 clusters representing 4 metabolites; HEI-2015 score was significantly associated with all. HEI-2015 score was associated with proline betaine [β = 0.12 (SE = 0.02); P = 4.70 × 10-13] and was inversely related to proline [β = -0.13 (SE = 0.02); P = 4.45 × 10-14], 1,5 anhydrosorbitol [β = -0.08 (SE = 0.02); P = 4.37 × 10-7] and unsaturated fatty acyl chains [β = 0.08 (SE = 0.02); P = 8.98 × 10-7]. Intake of total fruit, whole grains, and seafood and plant proteins was associated with proline betaine.
Diet quality is significantly associated with unsaturated fatty acyl chains, proline betaine, and proline. Further analysis may clarify the link between diet quality, metabolites, and pathogenesis of cardiometabolic disease.
The goal was to determine a pattern of metabolites associated with the Healthy Eating Index (HEI)-2015, which measures adherence to the Dietary Guidelines for Americans.
The analysis examined 3557 adult men and women from the longitudinal cohort Multiethnic Study of Atherosclerosis (MESA), without known cardiovascular disease and with complete dietary data. Fasting serum specimens and diet and demographic questionnaires were assessed at baseline. Untargeted 1H 1-dimensional nuclei magnetic resonance spectroscopy (600 MHz) was used to generate metabolomics and lipidomics. A metabolome-wide association study specified each spectral feature as outcomes, HEI-2015 score as predictor, adjusting for age, sex, race, and study site in linear regression analyses. Subsequently, hierarchical clustering defined the discrete groups of correlated nuclei magnetic resonance features associated with named metabolites, and the linear regression analysis assessed for associations with HEI-2015 total and component scores.
The sample included 50% women with an mean age of 63 years, with 40% identifying as White, 23% as Black, 24% as Hispanic, and 13% as Chinese American. The mean HEI-2015 score was 66. The metabolome-wide association study identified 179 spectral features significantly associated with HEI-2015 score. The cluster analysis identified 7 clusters representing 4 metabolites; HEI-2015 score was significantly associated with all. HEI-2015 score was associated with proline betaine [β = 0.12 (SE = 0.02); P = 4.70 × 10-13] and was inversely related to proline [β = -0.13 (SE = 0.02); P = 4.45 × 10-14], 1,5 anhydrosorbitol [β = -0.08 (SE = 0.02); P = 4.37 × 10-7] and unsaturated fatty acyl chains [β = 0.08 (SE = 0.02); P = 8.98 × 10-7]. Intake of total fruit, whole grains, and seafood and plant proteins was associated with proline betaine.
Diet quality is significantly associated with unsaturated fatty acyl chains, proline betaine, and proline. Further analysis may clarify the link between diet quality, metabolites, and pathogenesis of cardiometabolic disease.
摘要：
背景：不良饮食质量是2型糖尿病和心血管疾病的危险因素。然而,代谢物的知识表明遵守美国人膳食指南(2015版;DGA-15)是有限的.目标是确定与健康饮食指数-2015（HEI-2015）相关的代谢物模式，衡量对DGA的坚持。
方法：该分析从动脉粥样硬化的纵向队列多种族研究（MESA）中检查了3557名成年男性和女性，没有已知的心血管疾病和完整的饮食数据。空腹血清标本,在基线时评估饮食和人口统计学问卷.非靶向1HNMR1DNMR光谱(600MHz)用于产生代谢组学和脂质组学。一项全代谢组关联研究(MWAS)将每个光谱特征指定为结果，HEI-2015分数作为预测因子，调整年龄，性别,种族,和线性回归分析中的研究地点。随后，分层聚类定义了与命名代谢物相关的NMR特征的离散组,并评估了与HEI-2015总分和组分评分相关的线性回归分析.
结果：样本包括50％的女性，平均年龄为63岁，40%的人认为是白人，23%黑色,24%的西班牙裔和13%的美籍华人。HEI-2015平均得分为66。MWAS确定了179个与HEI-2015评分显著相关的光谱特征。聚类分析确定了代表4种代谢物的7个簇；HEI-2015评分与所有代谢物均显着相关。HEI-2015评分与脯氨酸甜菜碱（β0.12[0.02];p=4.70E-13）相关，与脯氨酸（β-0.13[0.02];p=4.45E-14）负相关，1,5脱水山梨醇（β-0.08[0.02];p=4.37E-07）和不饱和脂肪酰基链（β0.08[0.02];p=8.98E-07）。总水果摄入量，全谷物、海鲜和植物蛋白与脯氨酸甜菜碱有关。
结论：饮食质量与不饱和脂肪酰基链显著相关，脯氨酸甜菜碱,脯氨酸。进一步的分析可能会澄清饮食质量之间的联系，代谢物，和心脏代谢疾病的发病机制。
方法：该分析从动脉粥样硬化的纵向队列多种族研究（MESA）中检查了3557名成年男性和女性，没有已知的心血管疾病和完整的饮食数据。空腹血清标本,在基线时评估饮食和人口统计学问卷.非靶向1HNMR1DNMR光谱(600MHz)用于产生代谢组学和脂质组学。一项全代谢组关联研究(MWAS)将每个光谱特征指定为结果，HEI-2015分数作为预测因子，调整年龄，性别,种族,和线性回归分析中的研究地点。随后，分层聚类定义了与命名代谢物相关的NMR特征的离散组,并评估了与HEI-2015总分和组分评分相关的线性回归分析.
结果：样本包括50％的女性，平均年龄为63岁，40%的人认为是白人，23%黑色,24%的西班牙裔和13%的美籍华人。HEI-2015平均得分为66。MWAS确定了179个与HEI-2015评分显著相关的光谱特征。聚类分析确定了代表4种代谢物的7个簇；HEI-2015评分与所有代谢物均显着相关。HEI-2015评分与脯氨酸甜菜碱（β0.12[0.02];p=4.70E-13）相关，与脯氨酸（β-0.13[0.02];p=4.45E-14）负相关，1,5脱水山梨醇（β-0.08[0.02];p=4.37E-07）和不饱和脂肪酰基链（β0.08[0.02];p=8.98E-07）。总水果摄入量，全谷物、海鲜和植物蛋白与脯氨酸甜菜碱有关。
结论：饮食质量与不饱和脂肪酰基链显著相关，脯氨酸甜菜碱,脯氨酸。进一步的分析可能会澄清饮食质量之间的联系，代谢物，和心脏代谢疾病的发病机制。
