Abstract：
Proteins are important molecules involved in an immensely large number of biological processes. Being capable of manipulating proteins is critical for developing reliable and affordable techniques to analyze and/or detect them. Such techniques would enable the production of therapeutic agents for the treatment of diseases or other biotechnological applications (e.g., bioreactors or biocatalysis). Microfluidic technology represents a potential solution to protein manipulation challenges because of the diverse phenomena that can be exploited to achieve micro- and nanoparticle manipulation. In this review, we discuss recent contributions made in the field of protein manipulation in microfluidic systems using different physicochemical principles and techniques, some of which are miniaturized versions of already established macro-scale techniques.
摘要：
蛋白质是参与大量生物过程的重要分子。能够操纵蛋白质对于开发可靠且负担得起的技术来分析和/或检测它们至关重要。此类技术将能够生产用于治疗疾病或其他生物技术应用的治疗剂（例如，生物反应器或生物催化)。微流体技术代表了蛋白质操作挑战的潜在解决方案，因为可以利用多种现象来实现微和纳米颗粒操作。在这次审查中,我们讨论了使用不同的物理化学原理和技术在微流控系统中蛋白质操纵领域的最新贡献，其中一些是已经建立的宏观技术的小型化版本。
