PDF(Pubmed)
Abstract：
As one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor, tenderness, and juiciness of pork. Therefore, it is of great significance to study the mechanism of porcine IMF deposition. Previous research indicated that miR-149-5p promoted the proliferation of porcine intramuscular (IM) preadipocytes and decreased their ability to differentiate, albeit the exact mechanism of action is unknown. In vitro, foreign pigs showed increased miR-149-5p expression and reduced fat deposition when compared to Queshan Black pigs. This study conducted metabolomics and transcriptomics analyses of porcine IM preadipocytes overexpressing miR-149-5p to verify their effects on lipid formation. According to metabolomics analysis, the overexpression of miR-149-5p has significantly altered the lipid, organic acid, and organic oxygen metabolites of porcine IM preadipocytes. Specially speaking, it has changed 115 metabolites, including 105 up-regulated and 10 down-regulated ones, as well as the composition of lipid, organic acid, and organic oxygen metabolism-related metabolites. RNA-seq analysis showed that overexpression of miR-149-5p significantly altered 857 genes, of which 442 were up-regulated, and 415 were down-regulated, with enrichment to MAPK, IL-17, PI3K-Akt, and ErbB signaling pathways. We found that overexpression of miR-149-5p inhibited adipogenic differentiation by changing cAMP signaling pathway in porcine IM preadipocytes. In addition, the overexpression of miR-149-5p may affect the transport of Cu2+ by targeting ATP7A and inhibiting adipogenic differentiation. These findings elucidate the regulatory function of miR-149-5p in porcine IM preadipocytes, which may be a key target for controlling pork quality.
摘要：
作为养猪生产中的重要性状之一,肉品质具有重要的研究意义和价值。肌内脂肪(IMF)含量是影响猪肉品质的重要因素之一。许多实验研究表明，IMF含量与风味密切相关，压痛,和多汁的猪肉。因此,研究猪IMF沉积机理具有重要意义。先前的研究表明，miR-149-5p促进猪肌内（IM）前脂肪细胞的增殖并降低其分化能力，尽管确切的作用机制是未知的。体外，与QueshanBlack猪相比，外国猪显示miR-149-5p表达增加，脂肪沉积减少。这项研究对过表达miR-149-5p的猪IM前脂肪细胞进行了代谢组学和转录组学分析，以验证它们对脂质形成的影响。根据代谢组学分析,miR-149-5p的过表达显著改变了脂质，有机酸,猪IM前脂肪细胞的有机氧代谢产物。特别是,它改变了115种代谢物，包括105个上调和10个下调，以及脂质的组成，有机酸,和有机氧代谢相关的代谢产物。RNA-seq分析显示miR-149-5p的过表达显著改变了857个基因,其中442个被上调，415被下调，丰富到MAPK，IL-17,PI3K-Akt,和ErbB信号通路。我们发现miR-149-5p的过表达通过改变猪IM前脂肪细胞的cAMP信号通路来抑制脂肪分化。此外,miR-149-5p的过表达可能通过靶向ATP7A和抑制成脂分化来影响Cu2+的转运。这些发现阐明了miR-149-5p在猪IM前脂肪细胞中的调节功能,这可能是控制猪肉质量的关键目标。
