Abstract：
This study aims to identify an antemortem neck stab wound on a highly decomposed, headless and mutilated body by conventional hematoxylin-eosin (HE) staining combined with Ponceau-Victoria blue B staining (P-VB staining) and Masson staining. Specifically, a tissue sample was excised from the skin and muscle tissue at the junction of the normal and brownish discolored areas around the suspected stabbing tract of the left neck, in the upper and lower wound-clavicle-shoulder region. Conventional HE staining only provides a morphological and structural outline of the tissue, with both the injury hemorrhage and local connective tissue appearing eosinophilic pink. However, P-VB staining shows obvious contrast between the injury hemorrhage and connective tissue, with the former appearing yellow-green and the latter appearing orange-red. Similarly, Masson staining of the injury hemorrhage and connective tissue contrast clearly with purple-red and dark blue, respectively. Therefore, our study highlights that conventional HE staining with the combination of P-VB staining and Masson staining allowed for a clearer and corroborated identification of antemortem injury and hemorrhage from the stab wound in highly decomposed mutilated corpses.
摘要：
这项研究旨在确定一个高度分解的死前颈部刺伤，常规苏木精-伊红(HE)染色联合Ponceau-Victoria蓝B染色(P-VB染色)和Masson染色无头和残体。具体来说,从左颈部可疑刺伤道周围正常和褐色变色区域的交界处的皮肤和肌肉组织上切下组织样本，在上部和下部伤口-锁骨-肩部区域。常规HE染色仅提供组织的形态和结构轮廓，同时伴有损伤出血和局部结缔组织呈嗜酸性粉红色。然而,P-VB染色显示损伤出血和结缔组织之间的明显对比，前者呈黄绿色，后者呈橙红色。同样，Masson染色的损伤出血和结缔组织对比清楚，有紫红色和深蓝色，分别。因此,我们的研究强调,常规HE染色结合P-VB染色和Masson染色可以更清晰和确证地鉴定高度分解的尸体中的死前损伤和刺伤出血.
