Abstract：
Ticks are vectors of many zoonotic pathogens of clinical relevance, including Anaplasma and Rickettsia species. Since few data about these tick-borne pathogens are available in the Canary Islands, the aim of the present study was to screen their presence in questing and feeding ticks on the island of Tenerife. A total of 81 ticks was removed from six hedgehogs, and eight ticks were collected from the grass. PCR assays for tick species identification based on the LSU rRNA gene, and detection of Anaplasma spp. and Rickettsia spp. by targeting the 16s rRNA and gltA gene, respectively, were carried out. Rhipicephalus sanguineus and Haemaphysalis formosensis tick species were identified, with Rickettsia hoogstraalii detected in H. formosensis. In addition, Rickettsia helvetica and one unidentified Rickettsia species were detected. The overall prevalence of Rickettsia in ticks was 12.2%, and none harbored Anaplasma sp. This work constitutes the first identification in the Canary Islands of pathogenic R. helvetica and the species of unknown pathogenicity R. hoogstraalii in questing ticks. The clinical relevance of the pathogens identified highlights the need for studies with increased sample size and locations, including potential hosts, as well as warning the population about the relevance of ticks as vectors.
摘要：
蜱是许多临床相关的人畜共患病原体的载体，包括无性体和立克次体。由于加那利群岛几乎没有关于这些蜱传病原体的数据，本研究的目的是筛选它们在特内里费岛上寻找和喂养蜱的存在。从六只刺猬身上总共取出了81只蜱，从草地上收集了八个蜱。基于LSUrRNA基因的tick物种鉴定的PCR分析，和Anap理虫属的检测。和立克次体。通过靶向16srRNA和gltA基因，分别,进行了。已鉴定出血根虫和毛虫，在福莫森氏杆菌中检测到立克次体hoogstraalii。此外,检测到立克次体和一种未鉴定的立克次体。蜱中立克次体的总体患病率为12.2%,也没有人携带无性体。这项工作构成了加那利群岛中致病性R.helvetica和未知致病性R.hoogstraalii物种的首次鉴定。确定的病原体的临床相关性突出了需要增加样本量和位置的研究，包括潜在的宿主,以及警告人口关于蜱作为矢量的相关性。
