PDF(Pubmed)
Abstract：
Fusarium crown rot (FCR), mainly caused by Fusarium pseudograminearum, not only seriously threatens the yield and quality of wheat, but also endangers the health and safety of humans and livestock. Piriformospora indica is a root endophytic fungus that colonizes plant roots extensively and can effectively promote plant growth and improve plant resistance to biotic and abiotic stresses. In this study, the mechanism of FCR resistance mediated by P. indica in wheat was revealed from the phenylpropanoid metabolic pathway. The results showed that the colonization of P. indica significantly reduced the progression of wheat disease, the amount of F. pseudograminearum colonization, and the content of deoxynivalenol (DON) in wheat roots. RNA-seq suggested that P. indica colonization could reduce the number of differentially expressed genes (DEGs) in the transcriptome caused by F. pseudograminearum infection. The DEGs induced by the colonization of P. indica were partially enriched in phenylpropanoid biosynthesis. Transcriptome sequencing and qPCR indicated that the colonization of P. indica up-regulated the expression of genes involved in the phenylpropanoid biosynthesis pathway. The metabolome analysis indicated that the colonization of P. indica increased the metabolites\' accumulation in the phenylpropanoid biosynthesis. Consistent with transcriptome and metabolomic analysis, microscopic observations showed enhanced lignin accumulation in the roots of the Piri and Piri+Fp lines, most likely contributing to the arrested infection by F. pseudograminearum. These results suggested that P. indica increased resistance to F. pseudograminearum in wheat by inducing the phenylpropanoid pathway.
摘要：
镰刀菌冠腐病(FCR),主要由假镰刀菌引起，不仅严重威胁小麦的产量和品质，还会危害人类和牲畜的健康和安全。piriformosporaindicia是一种广泛定植于植物根系的根系内生真菌，能有效促进植物生长，提高植物对生物和非生物胁迫的抗性。在这项研究中,从苯丙素代谢途径揭示了小麦假单胞菌介导的FCR抗性机制。结果表明,迎草的定殖显著降低了小麦病害的进展,假赤霉F.定殖的数量，和小麦根中脱氧雪腐镰刀菌烯醇（DON）的含量。RNA-seq表明，印度假单胞菌定植可以减少假单胞菌感染引起的转录组中差异表达基因（DEG）的数量。在苯丙素类生物合成中部分富集了由紫花苜蓿定殖诱导的DEGs。转录组测序和qPCR表明，P的定植上调了与类苯丙烷生物合成途径有关的基因的表达。代谢组分析表明，在苯丙素类生物合成中，P.in的定植增加了代谢物的积累。与转录组和代谢组学分析一致，微观观察表明，Piri和PiriFp系的根部木质素积累增强，最有可能导致假赤霉F.的感染。这些结果表明，苦参通过诱导苯丙素途径增加了小麦对假赤霉病的抗性。
