Abstract：
Endometrial cancer is one of the common gynecological malignancies, and its incidence has been increasing year by year in recent years, raising higher requirements for its rapid diagnosis. In this article, gold nanorods (AuNRs) with localized surface plasmon resonance properties (LSPR) has used to prepare AuNRs-antibody to waveform protein (AuNRs-AntiVimentin) optical probes, and a new method has been constructed that could rapidly detect and identify endometrial cancer tissue sections by polarized light microscopy. AuNRs were prepared by seed growth method using gold chloride as raw material, and the morphology of AuNRs and the optical characteristics of AuNRs-AntiVimentin has characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), and zeta potential; immunohistochemistry (IHC) and AuNRs-AntiVimentin optical probes have used to detect clinical endometrial cancer, respectively. The AuNRs-AntiVimentin optical probe has been used to detect endometrial cancer tissue sections and found to have good bio-specificity, with no significant difference in the detection of AuNRs-AntiVimentin compared with conventional IHC techniques (p > .05). An optical probe generated by coupling AuNRs with Vimentin antibodies has been obtained to detect and identify endometrial cancer with simple operation and comparable effect to conventional IHC, providing a new method and idea for the rapid detection of endometrial cancer.
摘要：
子宫内膜癌是妇科常见的恶性肿瘤之一,近年来发病率逐年上升,对其快速诊断提出了更高的要求。在这篇文章中,具有局部表面等离子体共振特性（LSPR）的金纳米棒（AuNRs）已用于制备针对波形蛋白的AuNRs抗体（AuNRs-AntiVimentin）光学探针，并且已经构建了一种新的方法，可以通过偏振光显微镜快速检测和识别子宫内膜癌组织切片。以氯化金为原料,采用种子生长法制备AuNRs,通过透射电子显微镜(TEM)对AuNRs的形貌和AuNRs-AntiVimentin的光学特性进行了表征,紫外可见光谱(UV-Vis),和zeta电位；免疫组织化学（IHC）和AuNRs-AntiVimentin光学探针已用于检测临床子宫内膜癌，分别。AuNRs-AntiVimentin光学探针已用于检测子宫内膜癌组织切片，并发现具有良好的生物特异性，与常规IHC技术相比，AuNRs-AntiVimentin的检测没有显着差异（p>0.05）。已获得通过将AuNRs与波形蛋白抗体偶联而产生的光学探针，以简单的操作和与常规IHC相当的效果来检测和鉴定子宫内膜癌。为子宫内膜癌的快速检测提供了新的方法和思路。
