Abstract：
The long-term and excessive use of organophosphorus pesticides (OPs) leads to hazardous residues in the environment, which threatens human health to a considerable extent. Colorimetric methods can quickly and readily identify pesticide residue, but they still have various challenges in accuracy and stability. Herein, a smartphone-assisted and non-enzymatic colorimetric biosensor was constructed for rapid monitoring of multiple OPs based on the enhanced effect of aptamer on the catalytic ability of octahedral Ag2O. It was demonstrated that the aptamer sequence could enhance the affinity of colloidal Ag2O to chromogenic substrates, and accelerate the generation of oxygen radicals such as superoxide radical (·O2-) and singlet oxygen (1O2) from dissolved oxygen, thereby significantly increasing the oxidase activity of octahedral Ag2O. The color change of the solution can be easily converted to the corresponding RGB values by a smartphone for quantitative and rapid detection of multiple OPs. Hence, the smartphone-based and visual biosensor of multiple OPs was acquired with limit of detection of 10 μg L-1, 28 μg L-1 and 40 μg L-1 for isocarbophos, profenofos and omethoate, respectively. The colorimetric biosensor also exhibited good recoveries in several environmental and biological samples, showing that it may have broad application prospects for detecting OPs residues.
摘要：
有机磷农药(OPs)的长期和过度使用导致环境中的有害残留,这在很大程度上威胁着人类的健康。比色法能快速、方便地鉴别农药残留,但是它们在准确性和稳定性方面仍然面临各种挑战。在这里，基于适体对八面体Ag2O催化能力的增强作用，构建了智能手机辅助的非酶比色生物传感器，用于快速监测多种OPs。证明了适体序列可以增强胶体Ag2O对显色底物的亲和力，并加速从溶解氧中生成氧自由基，如超氧自由基（·O2-）和单线态氧（1O2），从而显著增加八面体Ag2O的氧化酶活性。溶液的颜色变化可以通过智能手机轻松转换为相应的RGB值，以定量和快速检测多个OP。因此，获得了基于智能手机的多种OPs的视觉生物传感器，其检测限为10μgL-1，28μgL-1和40μgL-1，profenofos和氧化乐果，分别。比色生物传感器在几种环境和生物样品中也表现出良好的回收率，表明其在OPs残留检测方面具有广阔的应用前景。
