PDF(Pubmed)
Abstract：
BACKGROUND: Nitric oxide (NO) exerts diverse effects on the cardiovascular system. Impairment of NO production plays a key role in cerebral and coronary artery spasm. We aimed to explore the predicting factors of radial artery spasm (RAS) and the association of eNOS gene polymorphism (Glu298Asp) with RAS during cardiac catheterization.
RESULTS: 200 patients underwent elective coronary angiography through a trans-radial approach. The subjects were genotyped to the Glu298Asp polymorphism (rs1799983) on the eNOS gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Our results showed that the subjects with the TT genotype and T allele were significantly more likely to develop radial artery spasms (OR = 12.5, 4.6, P < 0.001 respectively). TT genotype of eNOS Glu298Asp polymorphism, number of punctures, size of the radial sheath, radial tortuosity, and right radial access are independent predictors of radial spasm.
CONCLUSIONS: The eNOS (Glu298Asp) gene polymorphism is associated with RAS during cardiac catheterization in Egyptians. TT genotype of eNOS Glu298Asp polymorphism, number of punctures, size of the radial sheath, right radial access, and tortuosity are independent predictors of RAS during cardiac catheterization.
RESULTS: 200 patients underwent elective coronary angiography through a trans-radial approach. The subjects were genotyped to the Glu298Asp polymorphism (rs1799983) on the eNOS gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Our results showed that the subjects with the TT genotype and T allele were significantly more likely to develop radial artery spasms (OR = 12.5, 4.6, P < 0.001 respectively). TT genotype of eNOS Glu298Asp polymorphism, number of punctures, size of the radial sheath, radial tortuosity, and right radial access are independent predictors of radial spasm.
CONCLUSIONS: The eNOS (Glu298Asp) gene polymorphism is associated with RAS during cardiac catheterization in Egyptians. TT genotype of eNOS Glu298Asp polymorphism, number of punctures, size of the radial sheath, right radial access, and tortuosity are independent predictors of RAS during cardiac catheterization.
摘要：
背景：一氧化氮（NO）对心血管系统具有多种作用。NO产生的损害在大脑和冠状动脉痉挛中起关键作用。我们旨在探讨心脏导管插入术中桡动脉痉挛（RAS）的预测因素以及eNOS基因多态性（Glu298Asp）与RAS的相关性。
结果：200例患者经桡动脉途径行选择性冠状动脉造影。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）将受试者基因分型为eNOS基因上的Glu298Asp多态性（rs1799983）。我们的结果表明，具有TT基因型和T等位基因的受试者发生桡动脉痉挛的可能性更高（OR分别为12.5、4.6，P<0.001）。TT基因型eNOSGlu298Asp多态性,穿刺次数,径向护套的尺寸，径向弯曲，右径向通路是径向痉挛的独立预测因子。
结论：在埃及人的心脏导管插入术中，eNOS（Glu298Asp）基因多态性与RAS相关。TT基因型eNOSGlu298Asp多态性,穿刺次数,径向护套的尺寸，右径向入口,和弯曲是心脏导管插入术中RAS的独立预测因子。
结果：200例患者经桡动脉途径行选择性冠状动脉造影。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）将受试者基因分型为eNOS基因上的Glu298Asp多态性（rs1799983）。我们的结果表明，具有TT基因型和T等位基因的受试者发生桡动脉痉挛的可能性更高（OR分别为12.5、4.6，P<0.001）。TT基因型eNOSGlu298Asp多态性,穿刺次数,径向护套的尺寸，径向弯曲，右径向通路是径向痉挛的独立预测因子。
结论：在埃及人的心脏导管插入术中，eNOS（Glu298Asp）基因多态性与RAS相关。TT基因型eNOSGlu298Asp多态性,穿刺次数,径向护套的尺寸，右径向入口,和弯曲是心脏导管插入术中RAS的独立预测因子。
