Abstract:
A comparative assessment of AgNPs synthesized through three different routes viz. clove bud extract mediated AgNPs, sodium borohydride AgNPs and Glutathione (GSH) capped AgNPs for antioxidant and mosquito larvicidal activities was the major focus of the present study. The nanoparticles were characterized using UV-VIS spectrophotometry, dynamic light scattering (DLS), X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Characterization studies revealed the synthesis of stable, crystalline AgNPs measuring 28 nm, 7 nm and 36 nm for green, chemical and GSH-capped AgNPs respectively. FTIR analysis exhibited the surface functional moieties that were responsible for reduction, capping and stabilizing AgNPs. Antioxidant activity was found to be 74.11%, 46.62% and 58.78% for clove, borohydride and GSH-capped AgNPs respectively. Mosquito larvicidal bioactivity of AgNPs against Aedes aegypti IIIrd instar larvae depicted clove AgNPs being most effective (LC50-4.9 ppm, LC90-30.2 ppm) followed by GSH-capped (LC50-20.13 ppm, LC90-46.63 ppm) and borohydride AgNPs (LC50-13.43 ppm, LC90-160.19 ppm) after 24 h. Toxicity screening against aquatic model Daphnia magna revealed Clove mediated and GSH-capped AgNPs to be safer as compared to the borohydride AgNPs. It may be envisaged that green and capped AgNPs may be further explored for diverse biomedical and therapeutic applications.
摘要:
通过三种不同途径合成的AgNP的比较评估。丁香芽提取物介导的AgNPs,硼氢化钠AgNPs和谷胱甘肽(GSH)覆盖的AgNPs用于抗氧化和杀蚊子的活性是本研究的主要重点。使用紫外-可见分光光度法对纳米粒子进行表征,动态光散射(DLS),X射线衍射(XRD)场发射扫描电子显微镜(FE-SEM),透射电子显微镜(TEM)和傅里叶变换红外光谱(FTIR)分析。表征研究揭示了稳定的合成,晶体AgNPs测量28nm,7nm和36nm为绿色,化学和GSH封端的AgNPs。FTIR分析显示出负责还原的表面官能部分,加帽和稳定AgNPs。抗氧化活性为74.11%,丁香为46.62%和58.78%,分别为硼氢化物和GSH封端的AgNP。AgNPs对埃及伊蚊的杀幼虫生物活性显示丁香AgNPs最有效(LC50-4.9ppm,LC90-30.2ppm),然后是GSH封端(LC50-20.13ppm,LC90-46.63ppm)和硼氢化物AgNPs(LC50-13.43ppm,LC90-160.19ppm)24小时后。对水生模型大型水蚤的毒性筛选显示,与硼氢化物AgNP相比,丁香介导的和GSH封端的AgNP更安全。可以设想,绿色和封端的AgNP可以被进一步探索用于多种生物医学和治疗应用。