PDF(Pubmed)
Abstract：
Here we develop and characterize a dual-cross-linked pH-responsive hydrogel based on the carboxyethyl chitosan-oxidized sodium alginate (CAO) containing silver nanoparticles (Ag NPs) functionalized with tannic acid/red cabbage (ATR). This hybrid hydrogel is formed via covalent and non-covalent cross-linking. The adhesive strength measured in contact with cow skin and compression strength is measured more than 3 times higher than that of CAO. Importantly, the incorporation of 1 wt% ATR into CAO significantly enhances the compression strength of CAO from 35.1 ± 2.1 kPa to 97.5 ± 2.9 kPa. Moreover, the cyclic compression tests confirm significantly higher elastic behavior of CAO after the addition of ATR-functionalized NPs to CAO. The CAO/ATR hydrogel is pH-sensitive and indicated remarkable color changes in different buffer solutions. The CAO/ATR also shows improved hemostatic properties and reduced clotting time compared to the clotting time of blood in contact with CAO hydrogel. In addition, while CAO/ATR is effective in inhibiting the growth of both Gram-positive and Gram-negative bacteria, CAO is only effective in inhibiting the growth of Gram-positive bacteria. Finally, the CAO/ATR hydrogel is cytocompatible with L929 fibroblasts. In summary, the resulting CAO/ATR hydrogel shows promising results in designing and constructing smart wound bioadhesives with high cytocompatibility, antibacterial properties, blood coagulation ability, and fast self-healing properties.
摘要：
在这里，我们开发和表征双交联的pH响应水凝胶基于羧乙基壳聚糖氧化海藻酸钠（CAO）含银纳米颗粒（AgNP）用单宁酸/红卷心菜（ATR）官能化。该混合水凝胶通过共价和非共价交联形成。与牛皮肤接触时测得的粘合强度和压缩强度比CAO高3倍以上。重要的是,在CAO中加入1wt%的ATR显著提高了CAO的压缩强度，从35.1±2.1kPa提高到97.5±2.9kPa。此外，循环压缩测试证实了在将ATR官能化的NP添加到CAO之后CAO的显著更高的弹性行为。CAO/ATR水凝胶对pH敏感，并在不同的缓冲溶液中显示出明显的颜色变化。与与CAO水凝胶接触的血液的凝固时间相比,CAO/ATR还显示出改善的止血性质和减少的凝固时间。此外,虽然CAO/ATR在抑制革兰氏阳性和革兰氏阴性细菌的生长方面有效，CAO仅在抑制革兰氏阳性细菌的生长方面有效。最后,CAO/ATR水凝胶与L929成纤维细胞是细胞相容的。总之,所得的CAO/ATR水凝胶在设计和构建具有高细胞相容性的智能伤口生物粘合剂方面显示出有希望的结果，抗菌性能，凝血能力，和快速自我修复特性。
