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Abstract：
Biosensors capable of onsite and continuous detection of environmental and food pollutants and biomarkers are highly desired, but only a few sensing platforms meet the \"2-SAR\" requirements (sensitivity, specificity, affordability, automation, rapidity, and reusability). A fiber optic evanescent wave (FOEW) sensor is an attractive type of portable device that has the advantages of high sensitivity, low cost, good reusability, and long-term stability. By utilizing functional nucleic acids (FNAs) such as aptamers, DNAzymes, and rational designed nucleic acid probes as specific recognition ligands, the FOEW sensor has been demonstrated to be a general sensing platform for the onsite and continuous detection of various targets ranging from small molecules and heavy metal ions to proteins, nucleic acids, and pathogens. In this review, we cover the progress of the fluorescent FNA-based FOEW biosensor since its first report in 1995. We focus on the chemical modification of the optical fiber and the sensing mechanisms for the five above-mentioned types of targets. The challenges and prospects on the isolation of high-quality aptamers, reagent-free detection, long-term stability under application conditions, and high throughput are also included in this review to highlight the future trends for the development of FOEW biosensors capable of onsite and continuous detection.
摘要：
非常需要能够现场和连续检测环境和食品污染物和生物标志物的生物传感器，但只有少数传感平台满足“2-SAR”要求(灵敏度，特异性，负担能力，自动化,快速性,和可重用性)。光纤渐逝波（FOEW）传感器是一种有吸引力的便携式设备，具有高灵敏度的优点，低成本,良好的可重用性，和长期稳定。通过利用功能性核酸（FNA），如适体，DNAzyme,和合理设计的核酸探针作为特异性识别配体，FOEW传感器已被证明是一个通用的传感平台，用于现场和连续检测各种目标，从小分子和重金属离子到蛋白质，核酸,和病原体。在这次审查中,我们涵盖了自1995年首次报告以来基于荧光FNA的FOEW生物传感器的进展。我们专注于光纤的化学修饰以及对上述五种类型目标的传感机制。高质量适体分离的挑战和前景，无试剂检测，在应用条件下的长期稳定性，和高通量也包括在这篇评论中，以突出能够现场和连续检测的FOEW生物传感器发展的未来趋势。
