Abstract：
Commercially-supplied potato galactan (PG) is widely used as a model polysaccharide in various bioactivity studies. However, results using this galactan are not always consistent with the stated composition. Here, we assessed its composition by fractionating this commercial PG and purified its primary components: PG-A, PG-B and PG-Cp with weight-averaged molecular weights of 430, 93, and 11.3 kDa, respectively. PG-Cp consists of free β-1,4-galactan chains, whereas PG-A and PG-B are type I rhamnogalacturonans with long β-1,4-galactan side chains of up to 80 Gal residues and short β-1,4-galactan side chains of 0 to 3 Gal residues that display a \"trees in lawn\" pattern. Structures of these polysaccharides correlate well with their activities in terms of galectin-3 binding and gut bacterial growth assays. Our study clarifies the confusion related to commercial PG, with purified fractions serving as better model polysaccharides in bioactivity investigations.
摘要：
商业提供的马铃薯半乳聚糖（PG）在各种生物活性研究中被广泛用作模型多糖。然而,使用该半乳聚糖的结果并不总是与所述组合物一致。这里,我们通过分馏这种商业PG来评估其组成，并纯化其主要成分：PG-A，重均分子量为430、93和11.3kDa的PG-B和PG-Cp，分别。PG-Cp由游离的β-1,4-半乳聚糖链组成，而PG-A和PG-B是I型鼠李糖半乳糖醛类，具有多达80个Gal残基的长β-1,4-半乳聚糖侧链和0至3个Gal残基的短β-1,4-半乳聚糖侧链，显示“草坪中的树木”模式。就半乳糖凝集素-3结合和肠道细菌生长测定而言，这些多糖的结构与其活性密切相关。我们的研究澄清了与商业PG相关的混乱，纯化的馏分在生物活性研究中用作更好的模型多糖。
