PDF(Pubmed)
Abstract：
The objective of this study was to determine the effect of high-concentrate diets on the blood parameters and liver transcriptome of goats. Eighteen goats were allocated into three dietary treatments: the high level of concentrate (HC) group, the medium level of concentrate (MC) group, and the low level of concentrate (LC) group. The blood parameters and pathological damage of the gastrointestinal tract and liver tissues were measured. In hepatic portal vein blood, HC showed higher LPS, VFAs, and LA; in jugular vein blood, no significant differences in LPS, VFAs, and LA were recorded among groups (p > 0.05). Compared to the LC and MC groups, the HC group showed significantly increased interleukin (IL)-1β, IL-10, TNF-α, and diamine oxidase in jugular vein blood (p < 0.05). Liver transcriptome analysis discovered a total of 1269 differentially expressed genes (DEGs) among the three groups and most of them came from the HC vs. LC group. There were 333 DEGs up-regulated and 608 down-regulated in the HC group compared to the LC group. The gene ontology enrichment analysis showed that these DEGs were mainly focused on the regulation of triacylglycerol catabolism, lipoprotein particle remodeling, and cholesterol transport. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the liver of the HC group enhanced the metabolism of nutrients such as VFAs through the activation of AMPK and other signaling pathways and enhanced the clearance and detoxification of LPS by activating the toll-like receptor signaling pathway. A high-concentrate diet (HCD) can significantly promote the digestion of nutrients; the liver enhances the adaptability of goats to an HCD by regulating the expression of genes involved in nutrient metabolism and toxin clearance.
摘要：
这项研究的目的是确定高浓缩饮食对山羊血液参数和肝脏转录组的影响。将18只山羊分为三种饮食处理：高浓度浓缩物（HC）组，中浓度（MC）组，和低水平浓缩(LC)组。测定胃肠道和肝脏组织的血液参数和病理损害。在肝门静脉血液中，HC显示较高的LPS，VFA,和洛杉矶；在颈静脉血液中，LPS没有显著差异，VFA,各组间记录LA（p>0.05）。与LC和MC组相比，HC组白细胞介素（IL）-1β显著升高，IL-10,TNF-α,颈静脉血液中的二胺氧化酶（p<0.05）。肝脏转录组分析发现三组中共有1269个差异表达基因（DEGs），其中大部分来自HC与LC组。与LC组相比，HC组中有333个DEGs上调，而608个DEGs下调。基因本体富集分析表明,这些DEGs主要集中在三酰甘油分解代谢的调控上,脂蛋白颗粒重塑，和胆固醇运输。京都基因和基因组通路分析显示，HC组的肝脏通过激活AMPK和其他信号通路增强了VFA等营养物质的代谢，并通过激活toll样受体信号通路增强了LPS的清除和解毒作用。高浓缩饮食（HCD）可以显着促进营养物质的消化；肝脏通过调节参与营养代谢和毒素清除的基因的表达来增强山羊对HCD的适应性。
