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Abstract：
Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis-acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.
摘要：
小球藻是一种真核生物，可用作生产重组蛋白的工业宿主。在这项研究中,通过筛选在盐处理后上调的基因，从淡水物种小球藻PKVL7422中分离出盐诱导型启动子（SIP）。几个顺式作用元素，包括应激反应元素，在隔离的SIP中识别。此外，在SIP后克隆高斯荧光素酶基因，并将其转化到普通梭菌中，以测试该启动子的诱导性。对普通梭状芽胞杆菌转录组的重新检查表明，参与茉莉酸甲基（MeJA）合成的基因，赤霉素（GA），当用盐处理普通梭菌时，脱落酸（ABA）上调。此外，当用盐和MeJA处理转化的普通念珠菌时，重组荧光素酶的表达水平增加，GA,和ABA。这项研究代表了普通梭菌SIP的第一份报告，并强调了转化的微藻如何可用于重组蛋白的稳健表达。
