Abstract：
Invasive fungal infections may involve the brain and central nervous system (CNS), leading to often fatal meningitis in immunocompromised individuals. Recent technological advances have allowed us to move beyond studying the brain parenchyma to understanding the immune mechanisms of the meninges, the protective layer that surrounds the brain and spinal cord. Specifically, advanced microscopy techniques have enabled researchers to begin to visualize the anatomy of the meninges and the cellular mediators of meningeal inflammation. In this chapter, we describe how to make meningeal tissue mounts for imaging by confocal microscopy.
摘要：
侵袭性真菌感染可能涉及大脑和中枢神经系统（CNS），导致免疫功能低下的个体经常致命的脑膜炎。最近的技术进步使我们能够超越研究大脑实质，了解脑膜的免疫机制，围绕大脑和脊髓的保护层。具体来说,先进的显微镜技术使研究人员能够开始可视化脑膜的解剖结构和脑膜炎症的细胞介质。在这一章中,我们描述了如何制作用于共聚焦显微镜成像的脑膜组织支架。
