PDF(Pubmed)
Abstract：
Lipopolysaccharide (LPS) has previously been implicated in insulin resistance by generating an innate immune response and activating inflammatory cascades. Many studies have discovered a relationship between high levels of serum LPS and the advancement of diabetic microvascular problems, indicating that LPS may play a role in the control of critical signaling pathways connected to insulin resistance. The current study focused on signaling pathways linked to insulin resistance and explored probable mechanisms of LPS-induced insulin resistance in a murine model. It next looked at the effects of burdock, bee pollen, and -lipoic acid on LPS-induced inflammation and autoimmune defects in rats. LPS intoxication was induced via ip injection for one week in a dose of 10 mg/kg followed by α-lipoic acid, Burdock and bee pollen in an oral treatment for one month. Following that, biochemical and molecular studies were performed. The RNA expression of the regulating genes STAT5A and PTEN was measured. In addition, ATF-4 and CHOP as autophagy biomarkers were also subjected to mRNA quantification. The results demonstrated a considerable improvement in the -lipoic acid, Burdock, and bee pollen treated groups via modifying oxidative stress indicators as well as molecular ones. Furthermore, glucose concentration in serum and α-amylase were also improved upon treatment with the superiority of α-lipoic acid for modulating all estimated parameters. In conclusion: the results declared in the current study suggested that α-lipoic acid could regulate insulin resistance signaling pathways induced by LPS intoxication.
摘要：
脂多糖(LPS)先前通过产生先天性免疫应答和激活炎症级联反应而与胰岛素抵抗有关。许多研究已经发现高水平的血清LPS与糖尿病微血管问题的进展之间的关系,表明LPS可能在控制与胰岛素抵抗相关的关键信号通路中发挥作用。目前的研究集中在与胰岛素抵抗相关的信号通路，并探索了LPS诱导的小鼠模型中胰岛素抵抗的可能机制。接下来研究了牛磺酸的影响，蜂花粉,和-硫辛酸对LPS诱导的大鼠炎症和自身免疫缺陷的影响。通过腹膜内注射10mg/kg的剂量，然后用α-硫辛酸诱导LPS中毒一周，牛磺酸和蜂花粉口服治疗一个月。在此之后,进行了生化和分子研究.测量调节基因STAT5A和PTEN的RNA表达。此外,还对作为自噬生物标志物的ATF-4和CHOP进行mRNA定量。结果表明，硫辛酸有相当大的改善，牛犊,和蜂花粉处理组通过修改氧化应激指标以及分子指标。此外，血清中的葡萄糖浓度和α-淀粉酶在治疗后也得到改善，α-硫辛酸在调节所有估计参数方面具有优势。结论：本研究表明，α-硫辛酸可以调节LPS中毒诱导的胰岛素抵抗信号通路。
