PDF(Pubmed)
Abstract：
RNA granules are mesoscale assemblies that form in the absence of limiting membranes. RNA granules contain factors for RNA biogenesis and turnover and are often assumed to represent specialized compartments for RNA biochemistry. Recent evidence suggests that RNA granules assemble by phase separation of subsoluble ribonucleoprotein (RNP) complexes that partially demix from the cytoplasm or nucleoplasm. We explore the possibility that some RNA granules are nonessential condensation by-products that arise when RNP complexes exceed their solubility limit as a consequence of cellular activity, stress, or aging. We describe the use of evolutionary and mutational analyses and single-molecule techniques to distinguish functional RNA granules from \"incidental condensates.\"
摘要：
RNA颗粒是在不存在限制膜的情况下形成的中尺度组装体。RNA颗粒含有RNA生物发生和周转的因子，通常被认为代表RNA生物化学的专门区室。最近的证据表明，RNA颗粒通过亚可溶性核糖核蛋白（RNP）复合物的相分离而组装，该复合物部分地从细胞质或核质中分离。我们探索了当RNP复合物由于细胞活性而超过其溶解度极限时，某些RNA颗粒是非必需的缩合副产物的可能性。压力，或老化。我们描述了使用进化和突变分析和单分子技术来区分功能性RNA颗粒和“偶然凝聚物”。\"
