PDF(Pubmed)
Abstract：
Hepatic coccidiosis is an infectious and mortal disease that causes global economic losses in rabbits. The research aimed to assess the efficacy of Calotropis procure leaf extracts on the inhibition of Eimeria stiedae oocysts and to determine the optimal dosage for suppressing the parasite\'s infective phase. In this experiment, oocyst samples per milliliter were tested, and 6-well plates (2 mL) of 2.5% potassium dichromate solution containing 102 non-sporulated oocysts on Calotropis procera leaf extracts were exposed after 24, 48, 72, and 96 h, and the treatments were as follows: a nontreated control, 25%, 50%, 100%, and 150% of C. procera for oocyst activities. In addition, amprolium was utilized as a reference drug. The Calotropis procera was analyzed by GC-Mass, and results showed that the botanical extract contained 9 chemical components that were able to inhibit the oocysts of E. stiedae at 100% and 150% concentrations by about 78% and 93%, respectively. In general, an increase in the incubation period and a greater dose resulted in a decrease in the inhibition rate. The results showed that C. procera has an effective ability, inhibitory potential, and protective effect on the coccidian oocyst sporulation of E. stiedae. It can be used in the disinfection and sterilization of poultry and rabbit houses to get rid of Eimeria oocysts.
摘要：
肝球虫病是一种传染性和致命的疾病，在兔子中造成全球经济损失。该研究旨在评估Calotropis采购叶提取物对抑制艾美耳球虫卵囊的功效，并确定抑制寄生虫感染阶段的最佳剂量。在这个实验中,每毫升的卵囊样本进行了测试，和6孔板（2毫升）的2.5％重铬酸钾溶液含有102个非孢子化的卵囊在Calotropisprocera叶提取物后暴露24、48、72和96小时，治疗方法如下：未治疗的对照，25%,50%,100%,和150％的C.procera用于卵囊活动。此外,安丙啶被用作参考药物。Calotropisprocera通过GC-Mass分析，结果表明，植物提取物含有9种化学成分，能够以100％和150％的浓度抑制约78％和93％的卵囊。分别。总的来说,潜伏期的增加和更大的剂量导致抑制率降低。结果表明，C.procera具有有效的能力，抑制电位,对球虫卵囊孢子形成的保护作用。它可用于家禽和兔舍的消毒和灭菌，以去除艾美耳球虫卵囊。
