PDF(Pubmed)
Abstract：
In Chinese cabbage breeding, hybrids have made a terrific contribution due to heterosis, the superior performance of offspring compared to their inbred parents. Since the development of new, top-performing hybrids requires a large scale of human and material resources, the prediction of hybrid performance is of utmost interest to plant breeders. In our research, leaf transcriptome data from eight parents were used to investigate if they might be employed as markers to predict hybrid performance and heterosis. In Chinese cabbage, heterosis of plant growth weight (PGW) and heterosis of head weight (HW) were more obvious than other traits. The number of differential expression genes (DEGs) between parents was related to the PGW, length of the biggest outer leaf (LOL), leaf head height (LHH), leaf head width (LHW), HW, leaf number of head (LNH) and plant height (PH) of hybrids, and up-regulated DEGs number was also associated with these traits. Euclidean and binary distances of parental gene expression levels were significantly correlated with the PGW, LOL, LHH, LHW, HW and PH of hybrids. Additionally, there was a significant correlation between the parental expression levels of multiple genes involved in the ribosomal metabolic pathway and hybrid observations and heterosis in PGW, with the BrRPL23A gene showing the highest correlation with the MPH of PGW(r = 0.75). Therefore, leaf transcriptome data can preliminarily predict the hybrid performance and select parents in Chinese cabbage.
摘要：
在大白菜育种中，由于杂种优势，杂种做出了巨大的贡献，与近交父母相比，后代表现优异。新的发展以来,表现最好的混合动力车需要大量的人力和物力，杂种性能的预测是植物育种者最感兴趣的。在我们的研究中,来自八个亲本的叶片转录组数据用于研究它们是否可以用作预测杂种表现和杂种优势的标记。在大白菜中，植物生长重（PGW）和头重（HW）的杂种优势比其他性状更明显。亲本间差异表达基因(DEGs)的数量与PGW有关,最大外叶的长度（LOL），叶头高度（LHH），叶头宽度（LHW），HW,杂种的叶头数（LNH）和株高（PH），上调的DEGs数量也与这些性状相关。亲代基因表达水平的欧氏和二元距离与PGW显著相关,LOL,LHH,LHW,混合动力车的HW和PH。此外,在PGW中参与核糖体代谢途径的多个基因的亲本表达水平与杂种观察和杂种优势之间存在显着相关性，BrRPL23A基因与PGW的MPH相关性最高(r=0.75)。因此,叶片转录组数据可以初步预测大白菜的杂种表现和选择亲本。
