Abstract：
As chemical pesticides have caused serious environmental pollution, fungus-based biological control has become a developing alternative to chemical control. Here, we aimed to determine the molecular mechanism underlying how Metarhizium anisopliae facilitated invasive infection. We found that the fungus increased its virulence by downregulating glutathione S-transferase (GST) and superoxide dismutase (SOD) throughout termite bodies. Among 13 fungus-induced microRNAs throughout termite bodies, miR-7885-5p and miR-252b upregulation significantly downregulated several mRNAs in response to toxic substances to increase the fungal virulence [e.g., phosphoenolpyruvate carboxykinase (GTP) and heat shock protein homologue SSE1]. In addition, nanodelivered small interfering RNA of GST and SOD and miR-7885-5p and miR-252b mimics increased the virulence of the fungus. These findings provide new insights into the killing mechanism of entomopathogens and their utilization of the host miRNA machinery to reduce host defenses, laying the groundwork to enhance virulence of biocontrol agents for green pest management.
摘要：
由于化学农药对环境造成了严重的污染,基于真菌的生物防治已成为化学防治的一种发展中的替代品。这里,我们的目的是确定绿僵菌促进侵袭性感染的分子机制。我们发现该真菌通过下调整个白蚁体内的谷胱甘肽S-转移酶（GST）和超氧化物歧化酶（SOD）来提高其毒力。在整个白蚁体内的13种真菌诱导的microRNAs中，miR-7885-5p和miR-252b上调显着下调几个mRNA，以响应有毒物质增加真菌毒力[例如，磷酸烯醇丙酮酸羧激酶（GTP）和热休克蛋白同源物SSE1]。此外,纳米递送的GST和SOD的小干扰RNA以及miR-7885-5p和miR-252b模拟物增加了真菌的毒力。这些发现为昆虫病原体的杀伤机制及其利用宿主miRNA机制减少宿主防御提供了新的见解。为提高生物防治剂的毒力奠定了基础，用于绿色害虫管理。
