Abstract：
Cerebral stroke (stroke) is an acute cerebrovascular disease with high incidence and mortality. This study aimed to explore the association between single nucleotide polymorphisms (SNPs) of CYP4A22 and stroke risk in the Chinese Han population.
A total of 550 stroke patients and 545 healthy people were recruited. Four candidate SNPs (rs76011927 T/C, rs12564525 C/T, rs2056900 A/G and rs4926581 T/G) of CYP4A22 were screened. The association between CYP4A22 SNPs and stroke risk was assessed using genetic models and the relationship between SNPs and clinical biochemical indicators was analyzed by one-way analysis of variance (one-way ANOVA).
The overall analysis showed that rs12564525 could significantly reduce stroke risk only under the recessive model (OR = 0.72, 95% CI 0.53-0.99), but rs2056900 and rs4926581 were significantly associated with increased stroke risk under the homozygote (OR = 1.49, 95% CI 1.06-2.09; OR = 1.49, 95% CI 1.06-2.10), heterozygote (OR = 1.49, 95% CI 1.11-2.00; OR = 1.48, 95% CI 1.11-1.99), additive (OR = 1.22, 95% CI 1.03-1.45; OR = 1.22, 95% CI 1.03-1.45) and dominant (OR = 1.49, 95% CI 1.13-1.97; OR = 1.49, 95% CI 1.13-1.96) models (all p < 0.05). Subgroup analyses further indicated that rs2056900 and rs4926581 could significantly increase stroke risk in participants aged >63 years and females. In addition, high-density lipoprotein cholesterol (HDL-C) levels differed considerably among different genotypes of rs12564525, rs2056900 and rs4926581.
This study revealed that CYP4A22 SNPs are associated with stroke risk in the Chinese Han population, and in particular, rs2056900 and rs4126581 have a significant correlation with increased stroke risk.
A total of 550 stroke patients and 545 healthy people were recruited. Four candidate SNPs (rs76011927 T/C, rs12564525 C/T, rs2056900 A/G and rs4926581 T/G) of CYP4A22 were screened. The association between CYP4A22 SNPs and stroke risk was assessed using genetic models and the relationship between SNPs and clinical biochemical indicators was analyzed by one-way analysis of variance (one-way ANOVA).
The overall analysis showed that rs12564525 could significantly reduce stroke risk only under the recessive model (OR = 0.72, 95% CI 0.53-0.99), but rs2056900 and rs4926581 were significantly associated with increased stroke risk under the homozygote (OR = 1.49, 95% CI 1.06-2.09; OR = 1.49, 95% CI 1.06-2.10), heterozygote (OR = 1.49, 95% CI 1.11-2.00; OR = 1.48, 95% CI 1.11-1.99), additive (OR = 1.22, 95% CI 1.03-1.45; OR = 1.22, 95% CI 1.03-1.45) and dominant (OR = 1.49, 95% CI 1.13-1.97; OR = 1.49, 95% CI 1.13-1.96) models (all p < 0.05). Subgroup analyses further indicated that rs2056900 and rs4926581 could significantly increase stroke risk in participants aged >63 years and females. In addition, high-density lipoprotein cholesterol (HDL-C) levels differed considerably among different genotypes of rs12564525, rs2056900 and rs4926581.
This study revealed that CYP4A22 SNPs are associated with stroke risk in the Chinese Han population, and in particular, rs2056900 and rs4126581 have a significant correlation with increased stroke risk.
摘要：
背景：脑中风（stroke）是一种发病率和死亡率都很高的急性脑血管病。本研究旨在探讨CYP4A22基因单核苷酸多态性(SNPs)与中国汉族人群卒中风险的关系。
方法：共纳入550名脑卒中患者和545名健康人。四个候选SNP(rs76011927T/C，rs12564525C/T,筛选CYP4A22的rs2056900A/G和rs4926581T/G)。使用遗传模型评估CYP4A22SNP与卒中风险之间的关联，并通过单因素方差分析（单因素方差分析）分析SNP与临床生化指标之间的关系。
结果：总体分析表明，rs12564525仅在隐性模型下才能显着降低卒中风险（OR=0.72，95％CI0.53-0.99），但rs2056900和rs4926581与纯合子下卒中风险增加显著相关(OR=1.49,95%CI1.06-2.09;OR=1.49,95%CI1.06-2.10),杂合子(OR=1.49，95%CI1.11-2.00；OR=1.48，95%CI1.11-1.99)，加法模型(OR=1.22,95%CI1.03-1.45;OR=1.22,95%CI1.03-1.45)和显性模型(OR=1.49,95%CI1.13-1.97;OR=1.49,95%CI1.13-1.96)(均p<0.05)。亚组分析进一步表明，rs2056900和rs4926581可以显着增加年龄>63岁的参与者和女性的卒中风险。此外,高密度脂蛋白胆固醇（HDL-C）水平在rs12564525，rs2056900和rs4926581的不同基因型之间差异很大。
结论：这项研究表明，CYP4A22SNP与中国汉族人群的卒中风险相关。特别是,rs2056900和rs4126581与卒中风险增加有显著相关性。
方法：共纳入550名脑卒中患者和545名健康人。四个候选SNP(rs76011927T/C，rs12564525C/T,筛选CYP4A22的rs2056900A/G和rs4926581T/G)。使用遗传模型评估CYP4A22SNP与卒中风险之间的关联，并通过单因素方差分析（单因素方差分析）分析SNP与临床生化指标之间的关系。
结果：总体分析表明，rs12564525仅在隐性模型下才能显着降低卒中风险（OR=0.72，95％CI0.53-0.99），但rs2056900和rs4926581与纯合子下卒中风险增加显著相关(OR=1.49,95%CI1.06-2.09;OR=1.49,95%CI1.06-2.10),杂合子(OR=1.49，95%CI1.11-2.00；OR=1.48，95%CI1.11-1.99)，加法模型(OR=1.22,95%CI1.03-1.45;OR=1.22,95%CI1.03-1.45)和显性模型(OR=1.49,95%CI1.13-1.97;OR=1.49,95%CI1.13-1.96)(均p<0.05)。亚组分析进一步表明，rs2056900和rs4926581可以显着增加年龄>63岁的参与者和女性的卒中风险。此外,高密度脂蛋白胆固醇（HDL-C）水平在rs12564525，rs2056900和rs4926581的不同基因型之间差异很大。
结论：这项研究表明，CYP4A22SNP与中国汉族人群的卒中风险相关。特别是,rs2056900和rs4126581与卒中风险增加有显著相关性。
