Abstract:
To substitute petroleum-based materials with bio-based alternatives, microbial fermentation combined with inexpensive biomass is suggested. In this study Saccharina latissima hydrolysate, candy-factory waste, and digestate from full-scale biogas plant were explored as substrates for lactic acid production. The lactic acid bacteria Enterococcus faecium, Lactobacillus plantarum, and Pediococcus pentosaceus were tested as starter cultures. Sugars released from seaweed hydrolysate and candy-waste were successfully utilized by the studied bacterial strains. Additionally, seaweed hydrolysate and digestate served as nutrient supplements supporting microbial fermentation. According to the highest achieved relative lactic acid production, a scaled-up co-fermentation of candy-waste and digestate was performed. Lactic acid reached a concentration of 65.65 g/L, with 61.69% relative lactic acid production, and 1.37 g/L/hour productivity. The findings indicate that lactic acid can be successfully produced from low-cost industrial residues.
摘要:
用生物基替代品替代石油基材料,建议将微生物发酵与廉价的生物质相结合。在这项研究中,糖精水解产物,糖果工厂废料,探索了来自全规模沼气厂的消化物作为乳酸生产的基质。乳酸菌屎肠球菌,植物乳杆菌,和戊糖片球菌作为发酵剂培养物进行了测试。研究的细菌菌株成功利用了从海藻水解物和糖果废物中释放的糖。此外,海藻水解物和消化物作为支持微生物发酵的营养补充剂。根据实现的最高相对乳酸产量,进行了糖果废物和消化物的放大共发酵。乳酸浓度达到65.65g/L,相对乳酸产量为61.69%,和1.37g/L/小时生产率。研究结果表明,可以从低成本的工业残留物中成功生产乳酸。