PDF(Pubmed)
Abstract：
Hydrogen, oxygen, carbon, and nitrogen isotopes derived from three different strains of silkworms at different life stages involved in silkworm rearing, were measured to understand the fractionation characteristics of stable isotopes at different stages of silkworm development, and to trace the movement of these isotopes from food to larva to excrement and finally to silk. We found that silkworm strain had little effect on δ2H, δ18O and δ13C values. However, a large difference was found in the δ15N levels of newly-hatched silkworms between Jingsong Haoyue and Hua Kang No. 3 orthogonal strains, suggesting that the mating and egg laying differences may result in an inconsistent kinetic nitrogen isotope fractionation. The δ13C values of silkworm pupae and silkworm cocoon also displayed significant differences, suggesting that heavy carbon isotopes are greatly fractionated from the larva to the silk during cocoon formation. Overall, these results may be used to clarify the relationship between isotope fractionation and the ecological process of the Bombyx mori and expand our ability to resolve stable isotope anomalies at a small regional-scale level.
摘要：
氢,氧气,碳,和氮同位素来自三个不同的家蚕，这些家蚕处于不同的生命阶段，进行了测量，以了解家蚕发育不同阶段稳定同位素的分馏特征，追踪这些同位素从食物到幼虫到排泄物，最后到丝绸的运动。我们发现家蚕品系对δ2H的影响很小，δ18O和δ13C值。然而,京松浩月和华康号之间新孵化的蚕的δ15N水平存在很大差异。3个正交菌株，表明交配和产卵的差异可能导致动力学氮同位素分馏不一致。蚕蛹和蚕茧的δ13C值也表现出显著差异,这表明在茧形成过程中，重碳同位素从幼虫到蚕丝被大大分馏。总的来说,这些结果可用于阐明同位素分馏与家蚕生态过程之间的关系，并扩大我们在小区域范围内解决稳定同位素异常的能力。
