Abstract：
The rapid provision of blood products is life-saving for patients with massive hemorrhage. Ideally, RhD-negative blood products would be supplied to a woman of childbearing potential whose Rh type is unknown due to the risk of D-alloimmunization and the potential for hemolytic disease of the fetus and newborn to occur if RhD-positive blood products are transfused. Therefore, there is a need for a test that rapidly determines her RhD type. This study compared the RhD type determined using a rapid ABO and RhD test to the RhD type determined by an immunohematology reference laboratory.
After receiving ethics review board approval, 200 random, unique, deidentified patient samples that had undergone routine pretransfusion testing in an immunohematology reference laboratory using column agglutination technology were collected and tested using a rapid ABO and RhD test (Eldoncard Home kit 2511). The RhD typing results from these two methods were compared to determine the accuracy of the rapid ABO and RhD test.
The rapid ABO and RhD test produced results that were concordant with the transfusion service\'s results in 199/200 (99.5%) of cases, with a negative predictive value of 98.2% and 99.3% sensitivity. The single outlier was likely an RhD variant due to its serological characteristics.
These data indicate that this rapid ABO and RhD test could be used for the rapid determination of a patient\'s RhD type, perhaps even in the emergency department, which could guide the selection of blood products provided during their resuscitation.
After receiving ethics review board approval, 200 random, unique, deidentified patient samples that had undergone routine pretransfusion testing in an immunohematology reference laboratory using column agglutination technology were collected and tested using a rapid ABO and RhD test (Eldoncard Home kit 2511). The RhD typing results from these two methods were compared to determine the accuracy of the rapid ABO and RhD test.
The rapid ABO and RhD test produced results that were concordant with the transfusion service\'s results in 199/200 (99.5%) of cases, with a negative predictive value of 98.2% and 99.3% sensitivity. The single outlier was likely an RhD variant due to its serological characteristics.
These data indicate that this rapid ABO and RhD test could be used for the rapid determination of a patient\'s RhD type, perhaps even in the emergency department, which could guide the selection of blood products provided during their resuscitation.
摘要：
背景：快速提供血液制品可以挽救大出血患者的生命。理想情况下,RhD阴性血液制品将提供给有可能生育的妇女，其Rh类型未知，因为如果输注RhD阳性血液制品，会有D-同种免疫的风险以及胎儿和新生儿发生溶血性疾病的可能性。因此,需要一种快速确定她的RhD类型的测试。这项研究将使用快速ABO和RhD测试确定的RhD类型与免疫血液学参考实验室确定的RhD类型进行了比较。
方法：在获得伦理审查委员会批准后，200随机,独特,我们收集了在免疫血液学参考实验室使用柱凝集技术进行常规输血前检测的未识别患者样本,并使用快速ABO和RhD检测(EldoncardHomekit2511)进行检测.比较这两种方法的RhD分型结果，以确定快速ABO和RhD检验的准确性。
结果：在199/200例（99.5%）病例中，快速ABO和RhD试验产生的结果与输血服务的结果一致，阴性预测值为98.2%，敏感性为99.3%。由于其血清学特征，单个异常值可能是RhD变体。
结论：这些数据表明，这种快速ABO和RhD测试可用于快速确定患者的RhD类型，甚至在急诊室,这可以指导复苏期间提供的血液制品的选择。
方法：在获得伦理审查委员会批准后，200随机,独特,我们收集了在免疫血液学参考实验室使用柱凝集技术进行常规输血前检测的未识别患者样本,并使用快速ABO和RhD检测(EldoncardHomekit2511)进行检测.比较这两种方法的RhD分型结果，以确定快速ABO和RhD检验的准确性。
结果：在199/200例（99.5%）病例中，快速ABO和RhD试验产生的结果与输血服务的结果一致，阴性预测值为98.2%，敏感性为99.3%。由于其血清学特征，单个异常值可能是RhD变体。
结论：这些数据表明，这种快速ABO和RhD测试可用于快速确定患者的RhD类型，甚至在急诊室,这可以指导复苏期间提供的血液制品的选择。
