Abstract：
BACKGROUND: In plants, RNA silencing is an important conserved mechanism to regulate gene expression and combat against abiotic and biotic stresses. Dicer-like (DCL) and Argonaute (AGO) proteins and RNA-dependent RNA polymerase (RDR) are the core elements involved in gene silencing and their gene families have been explored in many plants. However, these genes and their responses to stresses have not yet been well characterized in adzuki bean.
RESULTS: A total of 11 AGO, 7 DCL and 6 RDR proteins were identified, and phylogenetic analyses of these proteins showed that they clustered into six, four and four clades respectively. The expression patterns of these genes in susceptible or resistant adzuki bean cultivars challenged with drought, bean common mosaic virus and Podosphaera xanthii infections were further validated by quantitative RT-PCR. The different responses of these proteins under abiotic and biotic stresses indicated their specialized regulatory mechanisms.
CONCLUSIONS: In this study, 24 genes of the DCL, AGO and RDR gene families in adzuki bean were identified, and the sequence characterization, structure of the encoded proteins, evolutionary relationship with orthologues in other legumes and gene expression patterns under drought and biotic stresses were primarily explored, which enriched our understanding of these genes in adzuki bean. Our findings provide a foundation for the comparative genomic analyses of RNA silencing elements in legume plants and further new insights into the functional complexity of RNA silencing in the response to various stresses in adzuki bean.
RESULTS: A total of 11 AGO, 7 DCL and 6 RDR proteins were identified, and phylogenetic analyses of these proteins showed that they clustered into six, four and four clades respectively. The expression patterns of these genes in susceptible or resistant adzuki bean cultivars challenged with drought, bean common mosaic virus and Podosphaera xanthii infections were further validated by quantitative RT-PCR. The different responses of these proteins under abiotic and biotic stresses indicated their specialized regulatory mechanisms.
CONCLUSIONS: In this study, 24 genes of the DCL, AGO and RDR gene families in adzuki bean were identified, and the sequence characterization, structure of the encoded proteins, evolutionary relationship with orthologues in other legumes and gene expression patterns under drought and biotic stresses were primarily explored, which enriched our understanding of these genes in adzuki bean. Our findings provide a foundation for the comparative genomic analyses of RNA silencing elements in legume plants and further new insights into the functional complexity of RNA silencing in the response to various stresses in adzuki bean.
摘要：
背景：在植物中，RNA沉默是调节基因表达和对抗非生物和生物胁迫的重要保守机制。Dicer样（DCL）和Argonaute（AGO）蛋白和RNA依赖性RNA聚合酶（RDR）是参与基因沉默的核心元件，其基因家族已在许多植物中得到探索。然而,这些基因及其对胁迫的反应在小豆中尚未得到很好的表征。
结果：总共11个AGO，鉴定出7种DCL和6种RDR蛋白,对这些蛋白质的系统发育分析表明，它们聚集为六个，分别有四个和四个分支。这些基因在受干旱挑战的易感或抗性小豆品种中的表达模式，通过定量RT-PCR进一步验证了豆类常见花叶病毒和Podosphaeraxanthii感染。这些蛋白质在非生物和生物胁迫下的不同反应表明了它们的专门调控机制。
结论：在这项研究中，DCL的24个基因，鉴定了小豆中的AGO和RDR基因家族，和序列表征，编码蛋白质的结构，主要探讨了与其他豆科植物直系同源物的进化关系以及干旱和生物胁迫下的基因表达模式，这丰富了我们对小豆中这些基因的理解。我们的发现为豆科植物中RNA沉默元件的比较基因组分析提供了基础，并进一步了解了小豆中RNA沉默在响应各种胁迫时的功能复杂性。
结果：总共11个AGO，鉴定出7种DCL和6种RDR蛋白,对这些蛋白质的系统发育分析表明，它们聚集为六个，分别有四个和四个分支。这些基因在受干旱挑战的易感或抗性小豆品种中的表达模式，通过定量RT-PCR进一步验证了豆类常见花叶病毒和Podosphaeraxanthii感染。这些蛋白质在非生物和生物胁迫下的不同反应表明了它们的专门调控机制。
结论：在这项研究中，DCL的24个基因，鉴定了小豆中的AGO和RDR基因家族，和序列表征，编码蛋白质的结构，主要探讨了与其他豆科植物直系同源物的进化关系以及干旱和生物胁迫下的基因表达模式，这丰富了我们对小豆中这些基因的理解。我们的发现为豆科植物中RNA沉默元件的比较基因组分析提供了基础，并进一步了解了小豆中RNA沉默在响应各种胁迫时的功能复杂性。
