Abstract：
The density and spread of tick vector species have increased throughout Europe in the last 30 years, leading to an increase of Lyme borreliosis cases, including in Slovenia. The aim of this study was to isolate Borrelia strains and determine the prevalence of B. burgdorferi sensu lato and B. miyamotoi in adults of Ixodes ricinus (Linnaeus) collected in 2019 in the two regions of the country (Coastal-Karst and Littoral-Inner Carniola) by cultivation and PCR. We isolated B. burgdorferi s.l. by culture method in 28/559 (5%) ticks from both regions. Culture-negative samples (531/559, i.e., 95%) were additionally tested by real-time PCR. In 155/531 (29.2%) PCR-positive samples, a fragment of flaB or glpQ was amplified and further sequenced to identify species of the Borrelia. Using both methods, cultivation and PCR, Borrelia spp. prevalence was 32.7% in the Coastal-Karst region and 33.0% in the Littoral-Inner Carniola region. Genotyping of the Borrelia spp. isolates revealed that 17/28 (60%) were B. garinii subtype Mlg2. Of all tick samples tested for B. miyamotoi 8/398 (2%) were PCR positive. Based on previous studies in these regions, we had expected more ticks to be infected with B. afzelii, but genotyping revealed that B. garinii was the most abundant.
摘要：
在过去的30年里，壁虱媒介物种的密度和传播在整个欧洲都在增加，导致莱姆病病例增加，包括在斯洛文尼亚。这项研究的目的是分离疏螺旋体菌株，并确定2019年在该国两个地区（沿海-喀斯特和沿海-内食肉）收集的蓖麻（Linnaeus）成虫中的B.burgdorferisensulato和B.miyamotoi的患病率。我们分离了B.burgdorferis.l.通过培养方法从两个地区的28/559（5％）蜱。培养阴性样本(531/559，即95%)通过实时PCR进行了额外测试。在155/531（29.2%）PCR阳性样本中，扩增了flaB或glpQ的片段，并进一步测序以鉴定疏螺旋体的物种。使用这两种方法，培养和PCR，疏螺旋体属。沿海-喀斯特地区的患病率为32.7%,沿海-内食肉地区的患病率为33.0%.疏螺旋体属的基因分型。分离株显示17/28(60%)是加氏芽孢杆菌Mlg2亚型。在所有测试miyamotoi芽孢杆菌8/398(2%)的蜱样品中,PCR呈阳性。根据这些地区以前的研究，我们原本预计会有更多的蜱虫感染阿兹利氏芽孢杆菌，但基因分型显示，B.garinii是最丰富的。
