PDF(Pubmed)
Abstract：
Babesia gibsoni is a tick-borne apicomplexan protozoan causing canine babesiosis. This parasite has diploid sexual reproduction in ticks, during which genetic exchanges can occur leading to increased genetic diversity, which is an important factor in adapting to environmental changes. Exploring the genetic variation of B. gibsoni population can provide a foundation for understanding the patterns of disease transmission and developing babesiosis control strategies. Partial 18S rRNA fragment sequences were obtained from 11 B. gibsoni isolates collected from different regions in China and 117 publicly available sequences were from 12 geographical areas including China. The genetic variation, demographic expansion and population structure were examined. A total of 34 haplotypes were identified among B. gibsoni populations. Analysis of molecular variance, pairwise Fst and structure analysis showed that high genetic variation within populations, low genetic differentiation and obvious mixture haplotype were apparent in a single continent, but higher genetic differentiation was detected across different continents. Neutrality tests implied that B. gibsoni populations had experienced population extension. These findings will contribute to understand the genetics and evolution of B. gibsoni and will be useful for formulating effective management strategies to prevent and control this parasite.
摘要：
gibsoni巴贝斯虫是引起犬巴贝斯虫病的tick传播的尖丛原生动物。这种寄生虫在蜱中具有二倍体有性繁殖，在此期间可以发生遗传交换，导致遗传多样性增加，这是适应环境变化的重要因素。探索吉布氏芽孢杆菌种群的遗传变异可以为了解疾病传播模式和制定巴贝斯虫病控制策略提供基础。从中国不同地区收集的11株B.gibsoni分离物中获得了部分18SrRNA片段序列，其中117个公开序列来自包括中国在内的12个地理区域。遗传变异,研究了人口膨胀和人口结构。在吉布氏芽孢杆菌种群中总共鉴定出34个单倍型。分子方差分析，成对的FST和结构分析表明，种群内的遗传变异较高，在单个大陆中明显存在低遗传分化和明显的混合单倍型，但是在不同的大陆上检测到更高的遗传分化。中立性检验表明B.gibsoni种群经历了种群扩展。这些发现将有助于了解吉布索尼的遗传学和进化，并将有助于制定有效的管理策略来预防和控制这种寄生虫。
