Abstract：
UNASSIGNED: Ex-vivo FCM is a novel digital optical technique that provides images of fresh tissues in a real-time fashion with magnification to subcellular details of a flattened unprocessed sample. Digital images are hematoxylin-eosin-like and can be shared and interpreted remotely. In urology, FCM has been successfully applied for prostate tissue interpretation, either during biopsy and radical prostatectomy. Possible applications of FCM may reflect those of frozen section analysis and can be extended to all fields in which the intra-operative microscopical control is advisable.
UNASSIGNED: This is an investigative prospective case series that aims to explore FCM feasibility in novel surgical settings and provide a depiction of FCM digital images in those fields. The definite purpose is to check the accuracy of surgical specimen during the following interventions: (a) trans-urethral resection of bladder tumors, to confirm the presence of muscular layer; (b) biopsy of a retroperitoneal mass, to check for the location and quality of cores; (c) training in robotic radical prostatectomy, to control surgical margins after a nerve sparing performed by a trainee. To this aim, we collected FCM images during seven surgical procedures. FCM findings were compared to those from the final histopathological analysis and the agreement was assessed.
UNASSIGNED: In all cases, FCM digital images were obtained in the OR. FCM was able to confirm the presence of muscular layer in TURB specimen, the presence of lymphomatous tissue, surgical margins at prostate specimen. FCM intra-operative interpretation was consistent with final histopathology in all cases.
UNASSIGNED: Ex vivo FCM may represent a novel approach to control the quality of specimens, likely to tailor surgical strategy in a real-time fashion. Moreover, digitalization represents a step toward the implementation of telepathology in clinical practice.
UNASSIGNED: This is an investigative prospective case series that aims to explore FCM feasibility in novel surgical settings and provide a depiction of FCM digital images in those fields. The definite purpose is to check the accuracy of surgical specimen during the following interventions: (a) trans-urethral resection of bladder tumors, to confirm the presence of muscular layer; (b) biopsy of a retroperitoneal mass, to check for the location and quality of cores; (c) training in robotic radical prostatectomy, to control surgical margins after a nerve sparing performed by a trainee. To this aim, we collected FCM images during seven surgical procedures. FCM findings were compared to those from the final histopathological analysis and the agreement was assessed.
UNASSIGNED: In all cases, FCM digital images were obtained in the OR. FCM was able to confirm the presence of muscular layer in TURB specimen, the presence of lymphomatous tissue, surgical margins at prostate specimen. FCM intra-operative interpretation was consistent with final histopathology in all cases.
UNASSIGNED: Ex vivo FCM may represent a novel approach to control the quality of specimens, likely to tailor surgical strategy in a real-time fashion. Moreover, digitalization represents a step toward the implementation of telepathology in clinical practice.
摘要：
■离体FCM是一种新颖的数字光学技术，它以实时方式提供新鲜组织的图像，并放大到扁平未加工样品的亚细胞细节。数字图像类似于苏木精-伊红，可以远程共享和解释。在泌尿科，FCM已成功应用于前列腺组织解释，活检和根治性前列腺切除术期间。FCM的可能应用可以反映冷冻切片分析的应用，并且可以扩展到建议进行术中显微控制的所有领域。
■这是一个调查性的前瞻性病例系列，旨在探索FCM在新型手术环境中的可行性，并提供FCM数字图像在这些领域的描述。明确的目的是在以下干预措施中检查手术标本的准确性：（a）经尿道切除膀胱肿瘤，确认肌肉层的存在；（b）腹膜后肿块的活检，检查核心的位置和质量；(C)机器人前列腺癌根治术的培训，在受训者进行神经保留后控制手术边缘。为了这个目标,我们在7次外科手术中收集了FCM图像。将FCM的发现与最终组织病理学分析的结果进行比较，并评估了一致性。
■在所有情况下，在OR中获得FCM数字图像。FCM能够确认TURB标本中肌肉层的存在，淋巴瘤组织的存在，前列腺标本的手术切缘。FCM术中解释与所有病例的最终组织病理学一致。
■离体FCM可能代表了一种控制标本质量的新颖方法，可能会实时定制手术策略。此外，数字化代表了在临床实践中实施远程病理学的一步。
■这是一个调查性的前瞻性病例系列，旨在探索FCM在新型手术环境中的可行性，并提供FCM数字图像在这些领域的描述。明确的目的是在以下干预措施中检查手术标本的准确性：（a）经尿道切除膀胱肿瘤，确认肌肉层的存在；（b）腹膜后肿块的活检，检查核心的位置和质量；(C)机器人前列腺癌根治术的培训，在受训者进行神经保留后控制手术边缘。为了这个目标,我们在7次外科手术中收集了FCM图像。将FCM的发现与最终组织病理学分析的结果进行比较，并评估了一致性。
■在所有情况下，在OR中获得FCM数字图像。FCM能够确认TURB标本中肌肉层的存在，淋巴瘤组织的存在，前列腺标本的手术切缘。FCM术中解释与所有病例的最终组织病理学一致。
■离体FCM可能代表了一种控制标本质量的新颖方法，可能会实时定制手术策略。此外，数字化代表了在临床实践中实施远程病理学的一步。
