METHODS: In accordance with CLSI guidelines, a disk diffusion test was carried out using 50 K. pneumoniae isolates obtained from various clinical samples. CT was computed in treated samples and compared to a susceptible ciprofloxacin strain (A111). The final finding is presented as the fold change in the target gene\'s expression in treated samples relative to a control sample (A111), normalized to a reference gene. As ΔΔCT = 0 and 2 to the power of 0 = 1, relative gene expression for reference samples is often set to 1 Results: The highest rates of resistance were recognized with cefotaxime (100%), cefuroxime (100%), cefepime (100%), levofloxacin (98%), trimethoprimsulfamethoxazole (80%), and gentamicin (72%), whereas imipenem (34%) had the lowest rates. Overexpression of acrA and acrB, oqxA and oqxB, regulators marA, soxS, and rarA were greater in ciprofloxacin-resistant isolates compared to the reference strain (strain A111). There was also a moderate connection between ciprofloxacin MIC and acrAB gene expression and a moderate connection between ciprofloxacin MIC and oqxAB gene expression.
CONCLUSIONS: This work provides a deeper knowledge of the role of efflux pump genes, particularly acrAB and oqxAB, as well as transcriptional regulators marA, soxS, and rarA, in bacterial resistance to ciprofloxacin.
方法:根据CLSI指南,我们使用从各种临床样本中获得的50株肺炎克雷伯菌进行了纸片扩散试验.在处理过的样品中计算CT,并与易感的环丙沙星菌株(A111)进行比较。最终发现以处理样品中靶基因表达相对于对照样品(A111)的倍数变化表示,标准化为参考基因。CT=0,20等于1,参考样品的相对基因表达通常设置为1。
结果:头孢噻肟的耐药率最高(100%),头孢呋辛(100%),头孢吡肟(100%),左氧氟沙星(98%),甲氧苄啶-磺胺甲恶唑(80%),和庆大霉素(72%),而亚胺培南(34%)的发病率最低。acrA和acrB的过表达,OQXA和OQXB,监管机构MarA,SOXS,与参考菌株(菌株A111)相比,环丙沙星耐药菌株中的rarA更高。环丙沙星MIC和acrAB基因表达之间也存在中度联系,环丙沙星MIC和oqxAB基因表达之间也存在中度联系。
结论:这项工作为外排泵基因的作用提供了更深入的知识,特别是acrAB和oqxAB,以及转录调节因子MarA,SOXS,还有RarA,细菌对环丙沙星的耐药性。