PDF(Pubmed)
Abstract：
The study included 32 women with PAS and 20 with normally implanted placenta as a control group. Vascular endothelial cell growth factor (VEGF), Soluble FMS Like Tyrosine Kinase (sFLT-1/sVEGFR1), and Endoglin (ENG) were measured in placenta tissue by ELISA. Granzyme B (GrzB) expression in trophoblastic and stromal mesenchymal cells was evaluated by immunohistochemistry. MAIT, NK, and NKT cells were assessed in blood and placenta by flow cytometry. Alterations were observed in levels of MAIT cells, NK cell subsets, and NKT cells in patients compared with controls. Several significant correlations were detected between these cells and GrzB scores, VEGF, ENG, and sFLT-1 levels. This is the first study analysing these cells in PAS patients and correlating their levels with changes in some angiogenic and antiangiogenic factors implicated in trophoblast invasion and with GrzB distribution in trophoblast and stroma. Interrelation between these cells probably plays an important role in pathogenesis of PAS.
摘要：
该研究包括32名PAS女性和20名正常植入胎盘的女性作为对照组。血管内皮细胞生长因子(VEGF),可溶性FMS样酪氨酸激酶(sFLT-1/sVEGFR1),通过ELISA测量胎盘组织中的Endoglin（ENG）。通过免疫组织化学评估滋养层和基质间充质细胞中的颗粒酶B（GrzB）表达。MAIT,NK,通过流式细胞术评估血液和胎盘中的NKT细胞。在MAIT细胞水平上观察到改变，NK细胞亚群，与对照组相比，患者的NKT细胞。在这些细胞和GrzB评分之间检测到几个显著的相关性，VEGF,ENG,和sFLT-1级别。这是第一项分析PAS患者中这些细胞的研究，并将其水平与某些与滋养细胞侵袭有关的血管生成和抗血管生成因子的变化以及滋养细胞和基质中的GrzB分布相关联。这些细胞之间的相互关系可能在PAS的发病机制中起重要作用。
