Abstract：
The protocol presented here is to derive, maintain, and differentiate human pluripotent stem cells into skeletal muscle progenitor/stem cells (myogenic progenitors) using a sphere-based culture approach. This sphere-based culture is an attractive method for maintaining progenitor cells due to their longevity and the presence of cell-cell interactions and molecules. Large numbers of cells can be expanded in culture using this method, which represents a valuable source for cell-based tissue modeling and regenerative medicine.
摘要：
这里提出的协议是推导，保持,并使用基于球体的培养方法将人多能干细胞分化为骨骼肌祖细胞/干细胞（生肌祖细胞）。由于祖细胞的寿命以及细胞-细胞相互作用和分子的存在，这种基于球体的培养是维持祖细胞的有吸引力的方法。使用这种方法可以在培养中扩增大量细胞，它代表了基于细胞的组织建模和再生医学的宝贵来源。
