{Reference Type}: Journal Article
{Title}: Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells.
{Author}: Reilly M;Robertson S;Suzuki M;
{Journal}: Methods Mol Biol
{Volume}: 2640
{Issue}: 0
{Year}: 2023
暂无{DOI}: 10.1007/978-1-0716-3036-5_12
{Abstract}: The protocol presented here is to derive, maintain, and differentiate human pluripotent stem cells into skeletal muscle progenitor/stem cells (myogenic progenitors) using a sphere-based culture approach. This sphere-based culture is an attractive method for maintaining progenitor cells due to their longevity and the presence of cell-cell interactions and molecules. Large numbers of cells can be expanded in culture using this method, which represents a valuable source for cell-based tissue modeling and regenerative medicine.