Abstract：
Dermal stem cells (DSCs), which are progenitor cells of melanocytes, are isolated from human foreskin and cultivated as mixed cultures containing both DSCs and fibroblasts in varying proportions. These contaminating fibroblasts may have an impact on the results of experimental studies and are a serious limitation for certain applications. The aim of the present study was to purify or enrich DSCs-an indispensable step towards future investigations. Applying different methods, we demonstrated that highly enriched DSCs with a good recovery rate can be obtained through positive selection with MACS® immunomagnetic cell sorting. These DSCs remain vital and proliferate constantly in culture, maintaining a high level of purity after enrichment. Other approaches such as treatment with Geneticin or selective detachment were not suitable to purify DSC-fibroblast co-cultures. Overall, enriched DSCs represent a novel and unique model to study the effects of UV radiation on the differentiation of DSCs into melanocytes and their potential relevance in the genesis of malignant melanoma.
摘要：
真皮干细胞(DSC),它们是黑素细胞的祖细胞，从人包皮中分离并培养为含有不同比例的DSC和成纤维细胞的混合培养物。这些污染的成纤维细胞可能对实验研究的结果有影响,并且对于某些应用是严重的限制。本研究的目的是纯化或富集DSC-这是未来研究不可或缺的一步。应用不同的方法，我们证明，通过MACS®免疫磁性细胞分选的阳性选择可以获得具有良好回收率的高度富集的DSC。这些DSCs在培养中仍然至关重要并不断增殖，富集后保持较高的纯度。其他方法如用遗传霉素处理或选择性分离不适合纯化DSC-成纤维细胞共培养物。总的来说,富集的DSCs代表了一种新颖而独特的模型，用于研究UV辐射对DSCs分化为黑素细胞的影响及其在恶性黑色素瘤发生中的潜在相关性。
