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Abstract：
Porcine reproductive and respiratory syndrome virus (PRRSV) causes clinical syndromes typified as reproductive disorders in sows and respiratory diseases in piglets. PRRSV remains one of the most prevalent pathogens affecting the pig industry, because of its complex infection profile and highly heterogeneous genetic and recombination characteristics. Therefore, a rapid and effective PRRSV detection method is important for the prevention and control of PRRS. With extensive in-depth research on PRRSV detection methods, many detection methods have been improved and promoted. Laboratory methods include techniques based on virus isolation (VI), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assays (IFA), immunoperoxidase monolayer assays (IPMA), polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), digital PCR (dPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR), metagenomic next-generation sequencing (mNGS), and other methods. This study reviews the latest research on improving the main PRRSV detection methods and discusses their advantages and disadvantages.
摘要：
猪繁殖与呼吸综合征病毒（PRRSV）引起临床综合征，典型为母猪生殖障碍和仔猪呼吸道疾病。PRRSV仍然是影响养猪业的最常见病原体之一，由于其复杂的感染特征和高度异质性的遗传和重组特征。因此,一种快速有效的PRRSV检测方法对PRRS的预防和控制具有重要意义。随着对PRRSV检测方法的广泛深入研究,许多检测方法得到了改进和推广。实验室方法包括基于病毒分离的技术（VI），酶联免疫吸附测定（ELISA），间接免疫荧光测定(IFA)，免疫过氧化物酶单层测定（IPMA），聚合酶链反应（PCR），定量实时PCR（qPCR），数字PCR(dPCR),环介导等温扩增(LAMP),重组酶聚合酶扩增（RPA），成簇的规则间隔短回文重复(CRISPR)，宏基因组下一代测序（mNGS），和其他方法。本研究综述了改进主要PRRSV检测方法的最新研究，并讨论了它们的优缺点。
