Abstract：
Super-resolution microscopy (SRM) technology that breaks the diffraction limit has revolutionized the field of cell biology since its appearance, which enables researchers to visualize cellular structures with nanometric resolution, multiple colors and single-molecule sensitivity. With the flourishing development of hardware and the availability of novel fluorescent probes, the impact of SRM has already gone beyond cell biology and extended to nanomedicine, material science and nanotechnology, and remarkably boosted important breakthroughs in these fields. In this review, we will mainly highlight the power of SRM in modern biomedical science, discussing how these SRM techniques revolutionize the way we understand cell structures, biomaterials assembly and how assembled biomaterials interact with cellular organelles, and finally their promotion to the clinical pre-diagnosis. Moreover, we also provide an outlook on the current technical challenges and future improvement direction of SRM. We hope this review can provide useful information, inspire new ideas and propel the development both from the perspective of SRM techniques and from the perspective of SRM\'s applications.
摘要：
自出现以来，打破衍射极限的超分辨率显微镜（SRM）技术彻底改变了细胞生物学领域，这使研究人员能够以纳米级分辨率可视化细胞结构，多种颜色和单分子灵敏度。随着硬件的蓬勃发展和新型荧光探针的问世,SRM的影响已经超越了细胞生物学，延伸到纳米医学，材料科学和纳米技术，并显著推动了这些领域的重大突破。在这次审查中,我们将主要强调SRM在现代生物医学中的力量，讨论这些SRM技术如何彻底改变我们理解电池结构的方式，生物材料组装以及组装的生物材料如何与细胞器相互作用，最后将其提升到临床预诊断。此外，我们还对SRM当前的技术挑战和未来的改进方向进行了展望。我们希望这篇评论能提供有用的信息，从SRM技术的角度和SRM应用的角度激发新的思想，推动发展。
