PDF(Pubmed)
Abstract：
The measurement of values of apparent equilibrium constants K\' for enzyme-catalyzed reactions involve a substantial number of critical details, neglect of which could lead to systematic errors. Here, interferences, impurities in the substances used, and failure to achieve equilibrium are matters of substantial consequence. Careful reporting of results is of great importance if the results are to have archival value. Thus, attention must be paid to the identification of the substances, specification of the reaction(s), the conditions of reaction, the definition of the equilibrium constant(s) and standard states, the use of standard nomenclature, symbols, and units, and uncertainties. This document contains a general discussion of various aspects of these equilibrium measurements as well as STRENDA (Standards for Reporting Enzymology Data) recommendations regarding the measurements and the reporting of results.
摘要：
酶催化反应的表观平衡常数K'值的测量涉及大量的关键细节。忽视这一点可能会导致系统误差。这里,干扰,所用物质中的杂质，而未能实现平衡是具有实质性后果的问题。如果结果要具有档案价值，那么仔细报告结果非常重要。因此，必须注意物质的识别，反应的规格，反应条件,平衡常数和标准状态的定义，使用标准命名法，符号,和单位,和不确定性。本文件包含对这些平衡测量的各个方面的一般性讨论，以及有关测量和结果报告的STRENDA（酶学数据报告标准）建议。
