Abstract：
In the study, melatonin, a known antioxidant pineal peptide was used as an additive in the tris-egg yolk glycerol-based semen extender in Hariana bull semen and post-thaw sperm characters were evaluated. In the study, Group I was a control without melatonin; and Group II, III, and IV were having 0.5 mM, 1 mM, and 2 mM melatonin/80 × 106 spermatozoa, respectively were treatment groups. Thirty-two semen ejaculates from 4 Hariana bulls were processed for freezing and post-thaw sperm characteristics were evaluated. Sperm motility, velocity, the viability with intact membrane, and total antioxidant capacity were markedly (P < 0.05) improved in Group IV compared to all other groups. The lipid peroxidation and total protein carbonylation were substantially (P < 0.05) decreased in Group IV compared to all other groups. The population of cryocapacitated, acrosome-reacted, and apoptotic-like spermatozoa were significantly (P < 0.05) decreased in Group IV. Further, the relative band intensity of 74 kDa protein and percent of spermatozoa showing positive immune reactivity to tyrosine-phosphorylated proteins was decreased in Group IV. The progesterone-receptor ligand binding, in vitro capacitation response, and Vanguard distance were markedly (P < 0.05) improved in Group IV. In summary- Group IV having 2 mM melatonin was found to be optimal in providing cryoprotective effects to Hariana bull spermatozoa after freezing-thawing and can be suitably used as a semen additive during semen cryopreservation.
摘要：
在研究中,褪黑激素,在Hariana公牛精液中，一种已知的抗氧化剂松果体肽被用作基于三卵黄甘油的精液补充剂中的添加剂，并评估了解冻后的精子特征。在研究中,第一组是没有褪黑激素的对照；第二组，III,IV有0.5mM，1mM,和2mM褪黑激素/80×106精子，分别为治疗组。对来自4头Hariana公牛的32个精液射精进行了冷冻处理，并评估了解冻后的精子特征。精子运动,速度,完整膜的生存能力，与所有其他组相比，IV组的总抗氧化能力显着提高（P<0.05）。与所有其他组相比,IV组的脂质过氧化和总蛋白羰基化显著降低(P<0.05)。冷冻的种群，顶体反应,Ⅳ组细胞凋亡样精子明显减少(P<0.05)。Further,第IV组74kDa蛋白的相对条带强度和对酪氨酸磷酸化蛋白显示阳性免疫反应性的精子百分比降低。孕激素受体配体结合，体外获能反应，第IV组Vanguard距离明显改善（P<0.05）。总之,发现具有2mM褪黑激素的第IV组在冻融后向Hariana公牛精子提供冷冻保护作用方面是最佳的,并且可以在精液冷冻保存期间合适地用作精液添加剂。
