Abstract：
Human menopausal gonadotrophin (hMG) has been reported to produce a comparable superovulatory response to that of follicle stimulating hormone (FSH). Furthermore, hMG has a long half-life as compared with FSH. The present study was designed to compare hMG administered once daily and FSH administered twice daily over a 4 - day period on superovulatory response of Suffolk ewes. During the mid-luteal phase, twenty-four Suffolk donor ewes received intravaginal sponges at day 0 for 12 days. The superovulatory regimens in the Control group (n = 12) and the Treatment group (n = 12) consisted of eight injections of FSH given at twice daily and four injections of hMG given at once daily, respectively. At day 13, the donor ewes were subjected to laparoscopic insemination. Embryos were recovered, classified, and transferred to recipient ewes at day 19. Pregnancy status was determined by ultrasound examination 40 days after transfer. Lambing rate was calculated after all the ewes had delivered. No significant differences were observed between the two groups in terms of the structures recovered, transferable embryos, degenerated embryos, unfertilized oocytes, pregnancy rate and lambing rate. The results showed that once daily injection of hMG can produce a comparable superovulatory response and embryo transfer outcomes to those obtained by twice daily injection of FSH over a 4 - day period. It is feasible that hMG is used to replace FSH and reduce the number of injection treatments in ovine superovulatory regimens.
摘要：
据报道，人类更年期促性腺激素（hMG）产生与卵泡刺激素（FSH）相当的超排卵反应。此外，与FSH相比,hMG具有长的半衰期。本研究旨在比较萨福克母羊在4天内每天一次给予hMG和每天两次给予FSH对超数排卵反应的影响。在黄体中期，24只萨福克供体母羊在第0天接受阴道内海绵，持续12天。对照组（n=12）和治疗组（n=12）的超排卵方案包括每天两次注射8次FSH和每天一次注射4次hMG，分别。在第13天,对供体母羊进行腹腔镜授精。胚胎恢复了,机密，并在第19天转移给接收母羊。转移后40天通过超声检查确定妊娠状态。在所有母羊交付后计算出羊率。两组在恢复的结构方面没有观察到显著差异,可转移的胚胎，退化的胚胎,未受精的卵母细胞，妊娠率和产仔数率。结果表明，每天一次注射hMG可以产生与每天两次注射FSH4天相当的超排卵反应和胚胎移植结果。在绵羊超排卵方案中，使用hMG替代FSH并减少注射治疗次数是可行的。
