Abstract：
The food color metanil yellow (Myl) is hazardous to several body systems. Evening primrose oil (EPO) was reported to have anti-inflammatory and anti-oxidant properties. The present work investigated the impact of Myl on the hepatic structure and function of rats and evaluated the protective effect of EPO. Forty adult male rats were divided into four groups: control, EPO (5 g/kg/day), Myl (200 mg/kg/day), and EPO- Myl group. Myl significantly increased liver enzymes, advanced glycation end products (AGE), oxidative stress parameters, pro-inflammatory cytokines, nuclear factor kappa B (NF-κB), and inducible nitric oxide synthase (iNOS). Blood vessels in the liver were dilated and congested, with cellular infiltration around them and associated with fibrosis. The hepatocytes were vacuolated and had dark nuclei. The immunohistochemical expression of iNOS, glial fibrillary acidic protein (GFAP), and Bax was significantly elevated. Ultrastructurally, the hepatocytes showed lipid droplets, irregular condensed nuclei with widened perinuclear space, dilated rER, mitochondria with destructed cristae, and multiple vacuoles. Dilated congested blood sinusoids and collagen fiber bundles were seen between hepatocytes. Interestingly, these alterations were less pronounced in rats co-administrated with EPO and Myl. In conclusion, EPO can protect liver against the toxic effects of Myl due to its anti-inflammatory and anti-oxidant activities.
摘要：
食用色素甲氨酚黄（Myl）对几种身体系统有害。据报道,月见草油(EPO)具有抗炎和抗氧化性质。本工作研究了Myl对大鼠肝脏结构和功能的影响，并评估了EPO的保护作用。将40只成年雄性大鼠分为四组：对照组，EPO（5g/kg/天），Myl（200mg/kg/天），和EPO-Myl基团。Myl显著增加肝酶，糖基化终产物（AGE），氧化应激参数，促炎细胞因子，核因子κB(NF-κB),和诱导型一氧化氮合酶（iNOS）。肝脏血管扩张并充血,周围有细胞浸润，并与纤维化有关。肝细胞是空泡的并且具有暗核。iNOS的免疫组织化学表达,胶质纤维酸性蛋白（GFAP），Bax显著升高。超微结构，肝细胞显示脂滴，核周空间变宽的不规则凝聚核，扩张的RER,线粒体有破损的cr，和多个液泡。肝细胞之间可见扩张的充血正弦和胶原纤维束。有趣的是,在与EPO和Myl共同给药的大鼠中，这些改变不太明显。总之,EPO具有抗炎和抗氧化活性，可以保护肝脏免受Myl的毒性作用。
