To assess the potential consequences of such pandemic-related deviations to normal practice, we surveyed nine international laboratories to determine if the characteristics or transplant outcomes of allogeneic stem cell donations differed in the immediate periods before and after the switch to routine cryopreservation.
Nine centers on two continents provided data for 72 HSC donations just before, and 71 just after, switching to cryopreservation for allogeneic HSC products. No statistically significant differences between the period before and after cryopreservation were noted for time from product collection to receipt, product temperature at receipt, or CD34+ cell viability at receipt. There was an indication of slower absolute neutrophil count recovery after cryopreservation was required (mean time of 15 vs. 17.6 days).
While there were no apparent changes to most parameters studied, there was an indication of slower neutrophil engraftment that will need to be examined in larger, longer term studies.
方法:为了评估这种与大流行相关的偏差对正常实践的潜在后果,我们调查了9个国际实验室,以确定异基因干细胞捐献的特征或移植结局在转换为常规冷冻保存前后的近期是否不同.
结果:两大洲的九个中心提供了之前72例HSC捐赠的数据,就在71号之后,转换为同种异体HSC产品的冷冻保存。从产品收集到接收的时间,在冷冻保存之前和之后的时间之间没有统计学上的显着差异,产品温度在收据,或收到时的CD34+细胞活力。需要冷冻保存后,有迹象表明中性粒细胞绝对计数恢复较慢(平均时间为15vs.17.6天)。
结论:虽然研究的大多数参数没有明显变化,有一个迹象表明,较慢的中性粒细胞植入,将需要在较大的检查,长期研究。