PDF(Pubmed)
Abstract：
Aphrodisiac herbal products originated from various plants including Mucuna species. In Thai folklore, Mucuna macrocarpa Wall. and M. pruriens (L.) DC. have long been consumed and utilized for their aphrodisiac properties. Consumption of these plants can lead to serious adverse effects caused by l-dopa. The plants have been legally banned for use as foods, dietary supplements, or nutraceuticals by the FDA of several countries. To protect consumers, methods for the identification of illicit plants or herbal products are needed. This study aimed to identify the selected twelve Mucuna species and examine the aphrodisiac herbal products containing M. macrocarpa and M. pruriens by using HPLC analysis of l-dopa coupled with DNA barcoding profiles of ITS, matK, rbcL, and trnH-psbA. The results showed that l-dopa could be found not only in the seeds of M. macrocarpa and M. pruriens but also in associated allied Mucuna species. Then, a DNA barcode was introduced to support in HPLC profiling to identify the plants. DNA barcodes of twelve Mucuna species found in Thailand were established and used to reconstruct a phylogenetic tree. In this study, ITS2 sequences showed the highest interspecific variability and could be used to differentiate all Mucuna species. The results of ITS2 sequence coupled with HPLC analysis revealed that all the purchased aphrodisiac products originated from M. pruriens only. Therefore, the integration of HPLC analysis and DNA barcoding profile was an efficient method for the identification of prohibited Mucuna species for safety monitoring of herbal supplements and protecting customer safety. Regulatory agencies should raise awareness and restrain the use of these commercial products.
摘要：
春药草药产品起源于包括Mucuna物种在内的各种植物。在泰国民间传说中，Mucunamacrocarpa墙。和M.pruriens（L.)DC。长期以来一直被消费和利用，因为它们的壮阳药特性。这些植物的消费可导致由左旋多巴引起的严重不利影响。这些植物已被法律禁止用作食品，膳食补充剂，或一些国家的FDA的营养品。为了保护消费者，需要识别非法植物或草药产品的方法。这项研究旨在鉴定选定的十二种Mucuna物种，并通过使用L-多巴的HPLC分析以及ITS的DNA条形码图谱来检查含有大孔菌和pruriens的壮阳草药产品，matK,rbcL,和trnH-psbA.结果表明，左旋多巴不仅可以在大叶M.moparpa和M.pruriens的种子中发现，而且可以在相关的Mucuna物种中发现。然后，引入DNA条形码以支持HPLC分析以鉴定植物。建立了在泰国发现的12种Mucuna物种的DNA条形码，并将其用于重建系统发育树。在这项研究中,ITS2序列显示出最高的种间变异性，可用于区分所有Mucuna物种。ITS2序列结合HPLC分析的结果表明，所有购买的壮阳药产品均仅来自pruriens。因此,HPLC分析和DNA条形码图谱的整合是鉴定违禁Mucuna物种的有效方法,用于草药补充剂的安全性监测和保护客户安全.监管机构应提高认识并限制使用这些商业产品。
