OBJECTIVE: This study aimed to investigate the effects of PRP glue treatment on EF and CN preservation in rats after CNSP.
METHODS: After prostatectomy, male Sprague-Dawley rats were treated with PRP glue, IC PRP injection, or their combination. The intracavernous pressure (ICP), mean arterial pressure (MAP), and CN preservation status in the rats were evaluated after 4 weeks. Results were corroborated using histology, immunofluorescence, and transmission electron microscopy.
RESULTS: The PRP glue-treated rats showed 100% CN preservation and significantly higher ICP responses (the ratio of maximum ICP to MAP (0.79 ± 0.09)) than the CNSP rats (the ratio of maximum ICP to MAP (0.33 ± 0.04)). PRP glue also significantly increased neurofilament-1 expression, indicating its positive effect on the CNs. Furthermore, this treatment significantly increased the expression of α-smooth muscle actin. Electron micrographs revealed that PRP glue preserved the myelinated axons and prevented atrophy of the corporal smooth muscle by maintaining the adherens junctions.
CONCLUSIONS: These results indicate that PRP glue is a potential solution for EF preservation by neuroprotection in patients with prostate cancer who are likely to undergo nerve-sparing radical prostatectomy.
目的:本研究旨在研究PRP胶处理对CNSP后大鼠EF和CN保存的影响。
方法:前列腺切除术后,雄性Sprague-Dawley大鼠用PRP胶治疗,ICPRP进样,或他们的组合。海绵体内压(ICP),平均动脉压(MAP),4周后评估大鼠的CN保存状态。结果用组织学证实,免疫荧光,和透射电子显微镜。
结果:与CNSP大鼠(最大ICP与MAP的比率(0.33±0.04))相比,PRP胶处理的大鼠显示出100%的CN保留和ICP响应(最大ICP与MAP的比率(0.79±0.09))。PRP胶也显著增加了神经丝-1的表达,表明其对CN的积极影响。此外,该治疗显着增加了α-平滑肌肌动蛋白的表达。电子显微照片显示,PRP胶保留了有髓鞘的轴突,并通过维持粘附连接来防止了体部平滑肌的萎缩。
结论:这些结果表明,PRP胶是可能接受保留神经的根治性前列腺切除术的前列腺癌患者通过神经保护保护EF的潜在解决方案。