关键词: Campylobacter infection Gram staining antigen testing gastroenteritis microscopic examination sensitivity and specificity

来  源:   DOI:10.1002/jgf2.596   PDF(Pubmed)

Abstract:
UNASSIGNED: Microscopic examination of stool samples can contribute to the early diagnosis of Campylobacter gastroenteritis. However, it is unclear whether the diagnostic performance is reliable when performed by physicians.
UNASSIGNED: This prospective study included fresh stool samples collected from patients with gastroenteritis between August 2018 and March 2020. The samples were used for microscopic examination through Gram staining. Two physicians, a clinical laboratory technician, and microbiologists performed the examinations. In addition, antigen tests (QuickNavi-Campylobacter; Denka Co., Ltd.) were evaluated for the samples collected between May 2019 and March 2020. Infection with Campylobacter spp. was confirmed when stool cultures or polymerase chain reaction tests provided positive results.
UNASSIGNED: Microscopic examination was performed on 205 samples, of which 46 (22.4%) were positive for Campylobacter spp. For the microscopic examination, the sensitivity and specificity were 53.5% and 98.1% for physician A, 46.7% and 96.2% for physician B, 63.0% and 100% for the clinical laboratory technician, and 67.4% and 100% for microbiologists, respectively. The antigen testing was evaluated in 131 of the 205 samples and showed a sensitivity of 93.3% and specificity of 99.0%.
UNASSIGNED: Microscopic examination of the stool samples showed high specificity. The sensitivity when the examinations were performed by the physicians was insufficient. The rapid antigen tests can reliably detect Campylobacter spp. in stool samples.
摘要:
未经证实:粪便样本的显微镜检查有助于弯曲杆菌胃肠炎的早期诊断。然而,由医师进行诊断时,诊断结果是否可靠尚不清楚.
UNASSIGNED:这项前瞻性研究包括2018年8月至2020年3月期间从胃肠炎患者收集的新鲜粪便样本。样品用于通过革兰氏染色的显微镜检查。两位医生,临床实验室技术员,微生物学家进行了检查。此外,抗原测试(QuickNavi-弯曲杆菌;DenkaCo.,有限公司)对2019年5月至2020年3月期间收集的样品进行了评估。弯曲杆菌感染。当粪便培养或聚合酶链反应测试提供阳性结果时,得到证实。
UNASSIGNED:对205个样品进行了显微镜检查,其中46例(22.4%)弯曲杆菌阳性。对于显微镜检查,A医师的敏感性和特异性分别为53.5%和98.1%,医生B的46.7%和96.2%,63.0%和100%为临床实验室技术员,微生物学家占67.4%和100%,分别。在205个样品中的131个样品中评估了抗原测试,显示出93.3%的灵敏度和99.0%的特异性。
UNASSIGNED:粪便样本的显微镜检查显示出高特异性。由医生进行检查时的灵敏度不足。快速抗原测试可以可靠地检测弯曲杆菌属。在粪便样本中。
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