Abstract：
This study was designed to investigate the hepatoprotective effects of Bacillus subtilis, a commensal bacterial species in the human gut, on ethanol-induced acute liver damage and the underlying mechanisms in mice. Male ICR mice challenged with three doses of ethanol (5.5 g/kg BW) exhibited a significant increase in serum aminotransferase activities and TNF-α level, liver fat accumulation, and activation of NF-κB signaling and NLRP3 inflammasome, which was suppressed by pretreatment with Bacillus subtilis. Besides, Bacillus subtilis inhibited acute ethanol-induced intestinal villi shortening and epithelial loss, the decline of protein levels of intestinal tight junction protein ZO-1 and occludin, and elevation of serum LPS level. Furthermore, the upregulation of mucin-2 (MUC2) and the downregulation of anti-microbial Reg3B and Reg3G levels induced by ethanol were repressed by Bacillus subtilis. Lastly, Bacillus subtilis pretreatment significantly increased the abundance of the intestinal Bacillus, but had no effects on the binge drinking-induced increase of Prevotellaceae abundance. These results demonstrate that Bacillus subtilis supplementation could ameliorate binge drinking-induced liver injury, and thus may serve as a functional dietary supplement for binge drinkers.
摘要：
本研究旨在研究枯草芽孢杆菌的保肝作用。人类肠道中的共生细菌，乙醇诱导小鼠急性肝损伤及其机制。用三种剂量的乙醇（5.5g/kgBW）攻击的雄性ICR小鼠的血清氨基转移酶活性和TNF-α水平显着增加，肝脏脂肪堆积,NF-κB信号和NLRP3炎性体的激活，被枯草芽孢杆菌预处理抑制。此外,枯草芽孢杆菌抑制急性乙醇诱导的肠绒毛缩短和上皮丢失，肠紧密连接蛋白ZO-1和闭塞蛋白的蛋白质水平下降，血清LPS水平升高。此外，枯草芽孢杆菌抑制了乙醇诱导的粘蛋白2（MUC2）的上调以及抗微生物Reg3B和Reg3G水平的下调。最后,枯草芽孢杆菌预处理显著增加了肠道芽孢杆菌的丰度,但对暴饮暴食引起的Prevotellaceae丰度增加没有影响。这些结果表明，补充枯草芽孢杆菌可以改善暴饮暴食引起的肝损伤，因此可以作为暴饮暴食者的功能性膳食补充剂。
